WB | 咨询技术 | Chicken |
IF | 咨询技术 | Chicken |
IHC | 咨询技术 | Chicken |
ICC | 技术咨询 | Chicken |
FCM | 咨询技术 | Chicken |
Elisa | 1/2000-1/5000 | Chicken |
Aliases | SERPINB14 |
WB Predicted band size | 43 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Chicken |
Immunogen | OVAL protein |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于OVAL(卵清蛋白)抗体的3篇模拟参考文献示例(注:文献为虚构示例,仅用于格式参考):
1. **文献名称**:*Production and characterization of monoclonal antibodies specific to ovalbumin for allergy research*
**作者**:Smith J, et al.
**摘要**:本研究开发了针对卵清蛋白(OVAL)的单克隆抗体,通过杂交瘤技术获得高特异性抗体,验证了其在ELISA和免疫印迹中的应用,为食物过敏诊断提供了新工具。
2. **文献名称**:*Role of anti-ovalbumin IgG in modulating immune responses in murine models*
**作者**:Lee H, et al.
**摘要**:通过小鼠模型探究抗OVAL的IgG抗体对Th2型免疫反应的调节作用,发现其可抑制嗜酸性粒细胞浸润,为哮喘和过敏性疾病治疗提供机制参考。
3. **文献名称**:*Affinity purification of ovalbumin using immobilized antibody columns: optimization and applications*
**作者**:Zhang R, et al.
**摘要**:研究基于抗OVAL抗体的亲和层析纯化方法,优化了洗脱条件并成功从复杂样本中高效分离卵清蛋白,提升了蛋白质组学分析的准确性。
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如需真实文献,建议通过PubMed或Google Scholar检索关键词:**"ovalbumin antibody" + "immunoassay"/"allergy"/"purification"**。
OVAL antibodies, commonly referring to antibodies targeting ovalbumin (OVA), are widely utilized in immunological research due to the protein’s role as a model antigen. Ovalbumin, a 45 kDa glycoprotein derived from chicken egg whites, is a well-characterized antigen often employed to study immune responses, hypersensitivity, and tolerance mechanisms. Its immunogenicity, stability, and ease of purification make it a standard tool in experimental immunology.
These antibodies are typically generated in host animals (e.g., rabbits, mice) through immunization with purified OVA. Polyclonal OVAL antibodies recognize multiple epitopes, while monoclonal variants target specific regions, enabling precise applications. They are instrumental in detecting OVA in assays like ELISA, Western blotting, or immunohistochemistry, and in tracking antigen-specific immune cells in flow cytometry.
In disease models, OVA is frequently used to induce allergic asthma or delayed-type hypersensitivity in mice. OVAL antibodies help quantify immune cell infiltration, cytokine profiles, or antigen uptake by dendritic cells, providing insights into adaptive immunity pathways. Additionally, OVA-antibody complexes are employed to study Fc receptor interactions or antigen presentation.
Commercial OVAL antibodies are widely available, often validated for specificity and cross-reactivity. Their utility spans basic research, vaccine development, and therapeutic studies, underscoring their foundational role in dissecting immune mechanisms.
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