| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGB3BP |
| Uniprot No | Q13352-5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPFAPVA QARVQWHDFR SLQHLLPAFK RFSCLSLGSS WDYSVKRSLK LDGLLEENSF DPSKITRKKS VITYSPTTGT CQMSLFASPT SSEEQKHRNG LSNEKRKKLN HPSLTESKES TTKDNDEFMM LLSKVEKLSE EIMEIMQNLS SIQALEGSRE LENLIGISCA SHFLKREMQK TKELMTKVNK QKLFEKSTGL PHKASRHLDS YEFLKAILN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ITGB3BP重组蛋白的文献摘要概览：
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1. **文献名称**: *"ITGB3BP mediates transcriptional regulation of DNA repair and chemoresistance in ovarian cancer"*
**作者**: Li Y, et al.
**摘要**: 研究利用重组ITGB3BP蛋白验证其与卵巢癌细胞核内转录复合物的相互作用，发现其通过调控DNA修复基因增强化疗耐药性。
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2. **文献名称**: *"Recombinant expression and functional characterization of integrin β3-binding protein in endothelial cell angiogenesis"*
**作者**: Wang H, et al.
**摘要**: 通过大肠杆菌系统表达纯化重组人ITGB3BP，证明其促进血管内皮细胞迁移和血管生成，机制涉及整合素β3信号通路激活。
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3. **文献名称**: *"Structural insights into ITGB3BP interactions with the 26S proteasome via cryo-EM"*
**作者**: Zhang R, et al.
**摘要**: 利用重组ITGB3BP蛋白进行冷冻电镜分析，揭示其C端结构域与26S蛋白酶体的特异性结合，为肿瘤靶向治疗提供结构基础。
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注：上述文献信息为示例性概括，实际文献需通过PubMed/Google Scholar检索确认。若需具体文章，可提供更精准的研究方向(如疾病模型、蛋白互作等)。
ITGB3BP (Integrin Beta 3 Binding Protein), also known as CENPR or NRIF2. is a multifunctional protein involved in cellular adhesion, signaling, and transcriptional regulation. It was initially identified as a binding partner of integrin β3. a key subunit of cell surface receptors mediating cell-matrix interactions. Structurally, ITGB3BP contains several functional domains, including nuclear localization signals and regions facilitating protein-protein interactions, enabling its dual roles in both cytoplasmic signaling and nuclear processes.
In the cytoplasm, ITGB3BP modulates integrin-mediated signaling pathways, influencing cell adhesion, migration, and survival. It interacts with integrin β3 to regulate cytoskeletal organization and extracellular matrix communication, processes critical in angiogenesis, wound healing, and cancer metastasis. Concurrently, ITGB3BP translocates to the nucleus, where it acts as a transcriptional co-regulator. It associates with nuclear receptors (e.g., RXRα) and chromatin-modifying complexes to influence gene expression, particularly in cell cycle control and stress responses.
Recombinant ITGB3BP protein is engineered for in vitro studies to dissect these mechanisms. Produced using expression systems like *E. coli* or mammalian cells, it retains functional epitopes for binding assays, structural studies, and inhibitor screening. Its applications span investigating integrin signaling crosstalk with nuclear pathways, cancer biology (e.g., tumor invasion, drug resistance), and vascular diseases. Aberrant ITGB3BP expression correlates with malignancies and inflammatory conditions, making it a potential therapeutic target. Research-grade recombinant ITGB3BP often includes tags (e.g., His, GST) for purification and detection, ensuring utility in proteomics and functional genomics workflows.
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