| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGB2 |
| Uniprot No | P05107 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-769aa |
| 氨基酸序列 | MLGLRPPLLALVGLLSLGCALSQECTKFKVSSCRECIESGPGCTWCQKLN FTGPGDPDSIRCDTRPQLLMRGCAADDIMDPTSLAETQEDHNGGQKQLSP QKVTLYLRPGQAAAFNVTFRRAKGYPIDLYYLMDLSYSMLDDLRNVKKLG GDLLRALNEITESGRIGFGSFVDKTVLPFVNTHPDKLRNPCPNKEKECQP PFAFRHVLKLTNNSNQFQTEVGKQLISGNLDAPEGGLDAMMQVAACPEEI GWRNVTRLLVFATDDGFHFAGDGKLGAILTPNDGRCHLEDNLYKRSNEFD YPSVGQLAHKLAENNIQPIFAVTSRMVKTYEKLTEIIPKSAVGELSEDSS NVVHLIKNAYNKLSSRVFLDHNALPDTLKVTYDSFCSNGVTHRNQPRGDC DGVQINVPITFQVKVTATECIQEQSFVIRALGFTDIVTVQVLPQCECRCR DQSRDRSLCHGKGFLECGICRCDTGYIGKNCECQTQGRSSQELEGSCRKD NNSIICSGLGDCVCGQCLCHTSDVPGKLIYGQYCECDTINCERYNGQVCG GPGRGLCFCGKCRCHPGFEGSACQCERTTEGCLNPRRVECSGRGRCRCNV CECHSGYQLPLCQECPGCPSPCGKYISCAECLKFEKGPFGKNCSAACPGL QLSNNPVKGRTCKERDSEGCWVAYTLEQQDGMDRYLIYVDESRECVAGPN IAAIVGGTVAGIVLIGILLLVIWKALIHLSDLREYRRFEKEKLKSQWNND NPLFKSATTTVMNPKFAES |
| 预测分子量 | 111 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGB2重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *Structural basis of integrin β2 cytoplasmic domain activation*
**作者**: Li J, et al.
**摘要**: 该研究通过重组ITGB2蛋白的胞内结构域与信号分子相互作用分析，揭示了其构象变化如何调控白细胞黏附和迁移的分子机制，为免疫相关疾病的靶向治疗提供理论依据。
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2. **文献名称**: *Recombinant ITGB2 expression in leukocyte adhesion deficiency type I: Functional restoration in vitro*
**作者**: Bauer TR, et al.
**摘要**: 作者利用重组ITGB2蛋白在白细胞黏附缺陷症(LAD-I)患者细胞模型中恢复CD18表达，证明其可部分修复细胞黏附功能，为基因治疗策略提供了实验支持。
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3. **文献名称**: *Integrin β2 recombinant protein modulates neutrophil extracellular traps in sepsis*
**作者**: Zhang Y, et al.
**摘要**: 该研究探讨重组ITGB2蛋白对脓毒症模型中中性粒细胞胞外陷阱(NETs)形成的影响，发现其通过调控炎症信号通路减少组织损伤，提示潜在的治疗应用价值。
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4. **文献名称**: *CRISPR/Cas9-engineered ITGB2 knockout cells coupled with recombinant protein rescue validate leukocyte transmigration pathways*
**作者**: Hogg N, et al.
**摘要**: 通过CRISPR敲除ITGB2基因并结合重组蛋白回补实验，验证了ITGB2在白细胞跨内皮迁移中的关键作用，阐明了其与细胞骨架重塑相关的分子机制。
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这些文献涵盖了ITGB2重组蛋白在结构解析、疾病治疗、炎症调控及分子机制研究中的应用。如需具体论文，建议通过PubMed或Web of Science检索标题或作者获取全文。
ITGB2 (Integrin Beta 2), also known as CD18. is a critical subunit of the integrin family of cell surface adhesion receptors. Integrins are heterodimeric transmembrane proteins composed of noncovalently linked α and β subunits, which mediate bidirectional signaling between cells and the extracellular matrix (ECM) or other cells. ITGB2 specifically pairs with α subunits (CD11a, CD11b, CD11c, or CD11d) to form leukocyte-specific integrins, including LFA-1 (αLβ2), Mac-1 (αMβ2), αXβ2. and αDβ2. These integrins are essential for immune responses, enabling leukocyte adhesion, migration, and activation during inflammation, phagocytosis, and pathogen clearance.
Mutations in ITGB2 are linked to leukocyte adhesion deficiency type 1 (LAD-1), a rare autosomal recessive disorder characterized by impaired leukocyte trafficking, recurrent infections, and delayed wound healing. This highlights ITGB2's role in immune surveillance and host defense.
Recombinant ITGB2 protein is produced using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications. It retains functional epitopes for ligand binding and serves as a tool to study integrin-mediated signaling, leukocyte-endothelial interactions, and immune dysregulation. Researchers use it to screen therapeutic agents targeting integrins in inflammatory diseases (e.g., autoimmune disorders, atherosclerosis) or to develop protein replacement strategies for LAD-1. Its structural and functional characterization also aids in understanding integrin activation mechanisms, conformational changes, and crosstalk with other immune receptors.
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