| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRF5 |
| Uniprot No | Q13568 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-498aa |
| 氨基酸序列 | MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHA TRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDF RLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEEL QRMLPSLSLTEDVKWPPTLQPPTLRPPTLQPPTLQPPVVLGPPAPDPSPL APPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDL EIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFP SPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPC ASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFG EEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDL KDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IRF5重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Structural basis for the recognition of DNA by the interferon regulatory factor 5"*
**作者**：Chen W. et al.
**摘要**：本研究通过重组表达并纯化人源IRF5蛋白，解析其DNA结合结构域的晶体结构，揭示了IRF5特异性识别干扰素刺激反应元件(ISRE)的关键氨基酸残基，为阐明其在天然免疫信号通路中的作用提供结构基础。
2. **文献名称**：*"Recombinant IRF5 regulates TLR9-dependent apoptosis and inflammatory cytokine production in lupus monocytes"*
**作者**：Barnes B.J. et al.
**摘要**：利用大肠杆菌系统表达重组IRF5蛋白，研究其在系统性红斑狼疮(SLE)患者单核细胞中的功能。发现IRF5通过激活TLR9信号通路促进促炎因子(如TNF-α、IL-6)分泌，并诱导细胞凋亡，提示其参与SLE的发病机制。
3. **文献名称**：*"Functional analysis of IRF5 polymorphisms using recombinant protein variants"*
**作者**：Krausgruber T. et al.
**摘要**：构建不同单核苷酸多态性(SNP)位点的IRF5重组蛋白突变体，发现特定SNP(如rs2004640)可增强IRF5的转录活性，导致干扰素信号通路过度激活，为遗传因素如何影响自身免疫疾病风险提供分子机制解释。
4. **文献名称**：*"IRF5-mediated signaling in dendritic cells requires post-translational modifications of the recombinant protein"*
**作者**：Salvador J.M. et al.
**摘要**：通过真核表达系统制备磷酸化修饰的重组IRF5蛋白，证明其磷酸化状态影响树突状细胞的成熟与抗原呈递功能，揭示翻译后修饰对IRF5调控免疫应答的关键作用。
(注：上述文献信息为示例性质，实际引用时需核对真实文献来源及作者信息。)
**Background of IRF5 Recombinant Protein**
Interferon Regulatory Factor 5 (IRF5) is a member of the IRF family of transcription factors, which play pivotal roles in regulating innate and adaptive immune responses. IRF5 is primarily involved in mediating signaling pathways triggered by Toll-like receptors (TLRs) and cytokine receptors, thereby influencing the production of pro-inflammatory cytokines (e.g., TNF-α, IL-6. IL-12) and type I interferons. It is highly expressed in immune cells such as macrophages, B cells, and dendritic cells, where it modulates cell differentiation, apoptosis, and pathogen defense. Dysregulation of IRF5 has been linked to autoimmune diseases (e.g., systemic lupus erythematosus, rheumatoid arthritis) and cancer, highlighting its dual role in immunity and disease pathogenesis.
Recombinant IRF5 protein is engineered through genetic cloning and expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce a purified, functional form of the protein for research and therapeutic applications. The recombinant protein typically retains key structural domains of IRF5. including the DNA-binding domain (DBD), intermediate association domain (IAD), and serine-rich domain (SRD), which are critical for its nuclear localization, dimerization, and transcriptional activity. Phosphorylation sites, essential for IRF5 activation, are often preserved to ensure biological relevance.
Researchers utilize IRF5 recombinant protein to study its interaction with DNA, co-regulators (e.g., NF-κB), and signaling pathways *in vitro*. It is also employed in drug discovery to screen inhibitors targeting IRF5-driven inflammation or oncogenesis. Additionally, recombinant IRF5 serves as an antigen for antibody production, aiding diagnostic and therapeutic developments. Its role in disease models further underscores its value in deciphering immune mechanisms and validating therapeutic targets.
Overall, IRF5 recombinant protein is a vital tool for elucidating IRF5 biology and advancing translational research in immunology and oncology.
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