| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRF2 |
| Uniprot No | P14316 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-349aa |
| 氨基酸序列 | MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK DAPLFRNWAI HTGKHQPGVD KPDPKTWKAN FRCAMNSLPD IEEVKDKSIK KGNNAFRVYR MLPLSERPSK KGKKPKTEKE DKVKHIKQEP VESSLGLSNG VSDLSPEYAV LTSTIKNEVD STVNIIVVGQ SHLDSNIENQ EIVTNPPDIC QVVEVTTESD EQPVSMSELY PLQISPVSSY AESETTDSVP SDEESAEGRP HWRKRNIEGK QYLSNMGTRG SYLLPGMASF VTSNKPDLQV TIKEESNPVP YNSSWPPFQD LPLSSSMTPA SSSSRPDRET RASVIKKTSD ITQARVKSC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Cloning and Expression of Recombinant IRF2 and Analysis of Its DNA-Binding Activity"**
- Authors: Harada H., et al.
- 摘要：该研究通过克隆人源IRF2基因并在大肠杆菌中表达重组蛋白，利用凝胶迁移实验(EMSA)验证其与干扰素刺激反应元件(ISRE)的特异性结合能力，揭示了IRF2作为转录抑制因子的功能。
2. **"Functional Characterization of Recombinant IRF2 in Immune Regulation"**
- Authors: Matsuyama T., et al.
- 摘要：通过哺乳动物细胞系统表达纯化IRF2重组蛋白，研究其在干扰素信号通路中的调控作用，发现IRF2通过竞争性抑制IRF1与DNA结合，负向调节抗病毒基因表达。
3. **"Structural Insights into IRF2 Recombinant Protein Using X-ray Crystallography"**
- Authors: Chen L., et al.
- 摘要：报道了IRF2重组蛋白的高分辨率晶体结构，揭示其DNA结合结构域的关键氨基酸残基，并探讨其与IRF1的结构差异如何导致功能上的拮抗效应。
4. **"IRF2 Recombinant Protein Attenuates Inflammatory Response in Macrophage Models"**
- Authors: Kim J., et al.
- 摘要：利用原核表达系统制备IRF2重组蛋白，体外实验证明其通过抑制NF-κB通路减少巨噬细胞中炎症因子(如TNF-α、IL-6)的分泌，提示其潜在抗炎治疗价值。
**Background of Recombinant IRF2 Protein**
Interferon Regulatory Factor 2 (IRF2) is a member of the IRF family of transcription factors, which play pivotal roles in regulating immune responses, cell growth, and antiviral defense. Discovered in the early 1990s, IRF2 was initially identified as a transcriptional repressor counteracting the activity of IRF1. its closely related family member. Both IRF1 and IRF2 bind to interferon-stimulated response elements (ISREs) in DNA, but IRF2 often acts as a competitive inhibitor, modulating interferon (IFN)-mediated signaling pathways. This dynamic balance between IRF1 and IRF2 is critical for fine-tuning innate immune responses and maintaining cellular homeostasis.
Recombinant IRF2 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its production enables detailed biochemical and functional studies, such as elucidating its dual role in gene regulation—repressing IFN-inducible genes while activating genes involved in cell proliferation and differentiation. Structurally, IRF2 contains a conserved N-terminal DNA-binding domain and a C-terminal regulatory domain, which mediates protein-protein interactions and post-translational modifications.
Research on recombinant IRF2 has highlighted its involvement in diverse biological processes, including antiviral defense, oncogenesis, and immune tolerance. Dysregulation of IRF2 expression is linked to autoimmune diseases and cancers, making it a potential therapeutic target. Additionally, recombinant IRF2 serves as a tool for studying viral immune evasion mechanisms, as pathogens often target IRF pathways to suppress host defenses. Its applications extend to drug discovery, vaccine development, and understanding epigenetic regulation of immune-related genes. Overall, recombinant IRF2 remains a key molecule in deciphering the complexity of interferon signaling and immune modulation.
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