| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRF1 |
| Uniprot No | P10914 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 11-276aa |
| 氨基酸序列 | WLEMQINSNQ IPGLIWINKE EMIFQIPWKH AAKHGWDINK DACLFRSWAI HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN KGSSAVRVYR MLPPLTKNQR KERKSKSSRD AKSKAKRKSC GDSSPDTFSD GLSSSTLPDD HSSYTVPGYM QDLEVEQALT PALSPCAVSS TLPDWHIPVE VVPDSTSDLY NFQVSPMPST SEATTDEDEE GKLPEDIMKL LEQSEWQPTN VDGKGYLLNE PGVQPTSVYG DFSCKE |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IRF1重组蛋白的3篇代表性文献摘要信息：
1. **文献名称**: "Recombinant Interferon Regulatory Factor 1 (IRF-1) is a DNA-binding transcription activator"
**作者**: Fujita T. et al.
**摘要**: 该研究首次报道了通过大肠杆菌表达系统重组表达人源IRF1蛋白，并验证其能够特异性结合干扰素基因启动子区域的DNA序列，激活下游基因转录，证实了IRF1在干扰素信号通路中的核心调控作用。
2. **文献名称**: "Purification and functional characterization of IRF1 in antiviral innate immunity"
**作者**: Tanaka N. et al.
**摘要**: 通过哺乳动物细胞表达系统纯化获得高活性IRF1重组蛋白，发现其能够直接激活干扰素刺激反应元件(ISRE)，并增强细胞抗病毒反应，揭示了IRF1在天然免疫中的关键分子机制。
3. **文献名称**: "Structural insights into IRF1-mediated DNA recognition through recombinant protein crystallography"
**作者**: Chen W. et al.
**摘要**: 研究利用昆虫细胞表达系统制备重组IRF1蛋白，通过X射线晶体学解析其DNA结合域的三维结构，阐明了IRF1与靶基因DNA结合的分子基础，为设计IRF1功能调控分子提供了结构依据。
注：以上为示例性内容，实际文献需根据具体研究检索确认。建议通过PubMed或Web of Science以"IRF1 recombinant protein"为关键词获取最新研究。
**Background of IRF1 Recombinant Protein**
Interferon Regulatory Factor 1 (IRF1) is a transcription factor belonging to the IRF family, which comprises nine members involved in immune responses, cell growth regulation, and oncogenesis. IRF1 was initially identified as a critical mediator of interferon (IFN) signaling, playing a pivotal role in antiviral defense and immune modulation. It binds to specific DNA sequences, such as interferon-stimulated response elements (ISREs), to regulate the expression of target genes, including those encoding cytokines, chemokines, and major histocompatibility complex (MHC) molecules.
IRF1 is ubiquitously expressed and activated by diverse stimuli, such as viral infections, IFN-γ, and DNA damage. Beyond its immunoregulatory functions, IRF1 participates in apoptosis, tumor suppression, and cellular differentiation. Dysregulation of IRF1 has been linked to autoimmune diseases, chronic inflammation, and cancer, underscoring its dual role as both a tumor suppressor and context-dependent oncogene.
Recombinant IRF1 protein is engineered through molecular cloning and expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional IRF1 for research and therapeutic applications. This protein retains the ability to bind DNA and regulate downstream genes, making it a valuable tool for studying IRF1-dependent pathways *in vitro* or *in vivo*. Researchers use it to investigate mechanisms of immune evasion, tumor progression, and host-pathogen interactions. Additionally, recombinant IRF1 holds potential in drug discovery, particularly for therapies targeting immune dysregulation or cancers with IRF1 pathway alterations.
Overall, IRF1 recombinant protein serves as a key reagent for dissecting the multifaceted roles of IRF1 in health and disease, bridging basic research with translational applications.
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