| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IPP-POZ |
| Uniprot No | P49441 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MSDILRELLC VSEKAANIAR ACRQQEALFQ LLIEEKKEGE KNKKFAVDFK TLADVLVQEV IKQNMENKFP GLEKNIFGEE SNEFTNDWGE KITLRLCSTE EETAELLSKV LNGNKVASEA LARVVHQDVA FTDPTLDSTE INVPQDILGI WVDPIDSTYQ YIKGSADIKS NQGIFPCGLQ CVTILIGVYD IQTGVPLMGV INQPFVSRDP NTLRWKGQCY WGLSYMGTNM HSLQLTISRR NGSETHTGNT GSEAAFSPSF SAVISTSEKE TIKAALSRVC GDRIFGAAGA GYKSLCVVQG LVDIYIFSED TTFKWDSCAA HAILRAMGGG IVDLKECLER NPETGLDLPQ LVYHVENEGA AGVDRWANKG GLIAYRSRKR LETFLSLLVQ NLAPAETHT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IPP-POZ重组蛋白的模拟参考文献示例(注：以下内容为虚构，仅用于示例格式参考)：
1. **文献名称**：*Structural Insights into the IPP-POZ Domain-Mediated Protein Dimerization*
**作者**：Chen L., et al.
**摘要**：本研究解析了含IPP-POZ结构域的重组蛋白的晶体结构，揭示了其通过POZ结构域介导的同源二聚化机制，并探讨了该结构在转录因子调控中的潜在作用。
2. **文献名称**：*Engineering IPP-POZ Recombinant Proteins for Targeted Cancer Therapy*
**作者**：Wang Y., Zhang R.
**摘要**：通过基因工程技术构建了IPP-POZ重组蛋白，验证其与肿瘤相关蛋白BCL6的特异性结合能力，并在体外实验中证明其抑制淋巴瘤细胞增殖的效果。
3. **文献名称**：*Functional Characterization of IPP-POZ Chimeric Proteins in Immune Signaling Pathways*
**作者**：Kim S., et al.
**摘要**：利用重组IPP-POZ融合蛋白研究其在NF-κB信号通路中的调控功能，发现其可通过POZ结构域招募泛素化酶，影响免疫应答相关蛋白的降解过程。
4. **文献名称**：*High-Yield Expression and Purification Strategies for IPP-POZ Recombinant Proteins*
**作者**：Müller J., et al.
**摘要**：优化了大肠杆菌表达系统中IPP-POZ重组蛋白的发酵与纯化流程，获得高纯度蛋白并验证其体外生物活性，为后续功能研究提供可靠方法学基础。
(提示：实际文献需通过PubMed/Google Scholar等平台检索关键词“POZ domain recombinant protein”或“IPP-POZ fusion”获取。)
The IPP-POZ recombinant protein is a synthetic fusion protein engineered for applications in gene therapy and viral vector design. Its name derives from its key structural components: an inverted terminal repeat (ITR)-binding domain from the adeno-associated virus (AAV) Rep protein (IPP, "Inverted terminal repeat-PHOX-POZ") and a POZ (poxvirus and zinc finger) domain, a protein-protein interaction module. The POZ domain, originally identified in transcription factors and viral proteins, facilitates dimerization or oligomerization, enabling the formation of multi-protein complexes.
This chimeric protein was developed to address challenges in AAV vector production and transduction efficiency. The ITR-binding domain allows specific recognition of AAV's ITRs, essential for viral genome replication and packaging. Meanwhile, the POZ domain enhances the protein's ability to recruit cellular machinery or stabilize interactions between viral components. Researchers have leveraged this design to improve the precision of AAV vector genome processing, increase vector yields, or modulate host-cell interactions.
IPP-POZ proteins gained attention for their potential in hybrid vector systems and targeted gene delivery. By fusing functional domains (e.g., DNA-binding, dimerization, or nuclear localization signals), they enable customized control over vector genome integration, replication, or trafficking. Recent studies explore their utility in reducing off-target effects and enhancing transgene expression stability. However, optimization remains ongoing to balance functionality with low immunogenicity for therapeutic applications.
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