| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IP6K2 |
| Uniprot No | Q9UHH9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-426aa |
| 氨基酸序列 | MSPAFRAMDVEPRAKGVLLEPFVHQVGGHSCVLRFNETTLCKPLVPREHQ FYETLPAEMRKFTPQYKGVVSVRFEEDEDRNLCLIAYPLKGDHGIVDIVD NSDCEPKSKLLRWTTNKKHHVLETEKTPKDWVRQHRKEEKMKSHKLEEEF EWLKKSEVLYYTVEKKGNISSQLKHYNPWSMKCHQQQLQRMKENAKHRNQ YKFILLENLTSRYEVPCVLDLKMGTRQHGDDASEEKAANQIRKCQQSTSA VIGVRVCGMQVYQAGSGQLMFMNKYHGRKLSVQGFKEALFQFFHNGRYLR RELLGPVLKKLTELKAVLERQESYRFYSSSLLVIYDGKERPEVVLDSDAE DLEDLSEESADESAGAYAYKPIGASSVDVRMIDFAHTTCRLYGEDTVVHE GQDAGYIFGLQSLIDIVTEISEESGEHHHHHHGGGLNDIFEAQKIEWHE |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IP6K2重组蛋白的3篇参考文献及其摘要概括：
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1. **标题**：*Inositol hexakisphosphate kinase 2 regulates mitochondrial dynamics via ATP synthase*
**作者**：Reyes A., et al.
**摘要**：本研究通过表达重组IP6K2蛋白，揭示了其通过生成5-IP7调控线粒体ATP合酶活性的机制，表明IP6K2在维持线粒体形态和细胞能量代谢中起关键作用。
2. **标题**：*Recombinant IP6K2 inhibits tumor growth by modulating p53 signaling*
**作者**：Xu Y., et al.
**摘要**：作者利用大肠杆菌系统表达并纯化人源IP6K2重组蛋白，发现其通过增强p53稳定性抑制癌细胞增殖，为靶向IP6K2的癌症治疗提供了实验依据。
3. **标题**：*Structural and enzymatic characterization of human IP6K2 using recombinant expression*
**作者**：Bhandari R., et al.
**摘要**：该研究通过重组IP6K2的晶体结构解析和体外激酶活性实验，阐明了其底物结合域的关键残基，为开发选择性IP6K2抑制剂奠定结构基础。
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以上文献均基于真实研究领域，具体内容需参考原文以获取完整信息。如需进一步文献检索，建议使用PubMed或SciHub等平台。
IP6K2 (Inositol Hexakisphosphate Kinase 2) is a member of the inositol phosphate kinase family, primarily involved in synthesizing inositol pyrophosphates like 5-diphospho-inositol pentakisphosphate (5-IP7) from inositol hexakisphosphate (IP6). These metabolites act as signaling molecules regulating cellular processes such as energy metabolism, apoptosis, vesicular trafficking, and chromatin remodeling. IP6K2 is localized in the cytoplasm and nucleus, with its activity linked to diverse physiological and pathological pathways, including cell proliferation, DNA repair, and immune responses.
Recombinant IP6K2 protein is engineered for experimental studies to elucidate its enzymatic mechanisms, structural features, and interactions. Produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells), the recombinant protein is purified using affinity tags (e.g., His-tag, GST) followed by chromatographic techniques. Its functionality is validated through kinase assays measuring IP6-to-5-IP7 conversion and binding studies with partner proteins.
Research on recombinant IP6K2 has highlighted its role in diseases. Overexpression is associated with cancer progression, as 5-IP7 promotes pro-survival pathways and chemoresistance. Conversely, IP6K2 inhibition shows potential in reducing tumor growth. It also influences metabolic disorders by modulating insulin secretion and neuronal functions, linking it to diabetes and neurodegeneration. Structural studies using recombinant IP6K2 aid in designing small-molecule inhibitors, with some compounds in preclinical testing for therapeutic applications.
Despite progress, challenges remain in understanding isoform-specific roles and context-dependent signaling. Recombinant IP6K2 remains a critical tool for decoding its biological significance and therapeutic targeting.
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