| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/15-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HPP1; ula-1; APPBP1; A-116A10.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NAE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NAE1抗体的3篇代表性文献的模拟示例(注：文献信息为示例，非真实存在)：
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1. **文献名称**：*NAE1 Expression Correlates with Tumor Progression in Colorectal Cancer*
**作者**：Zhang Y, et al.
**摘要**：本研究通过免疫组化(使用NAE1特异性抗体)分析结直肠癌组织中NAE1蛋白的表达水平，发现NAE1高表达与肿瘤分期和转移显著相关，提示其可作为潜在预后标志物。
2. **文献名称**：*Targeting NEDDylation: Mechanistic Insights into MLN4924-Induced NAE1 Inhibition*
**作者**：Brown K, et al.
**摘要**：研究利用NAE1抗体进行Western blot和免疫共沉淀实验，证实MLN4924通过抑制NAE1活性阻断Cullin蛋白的neddylation修饰，从而诱导癌细胞周期停滞和凋亡。
3. **文献名称**：*NAE1 Antibody-Based Detection of Neddylation Dysregulation in Breast Cancer*
**作者**：Lee S, et al.
**摘要**：通过NAE1抗体检测乳腺癌细胞系和组织样本，发现NAE1表达与neddylation通路的异常激活相关，且其沉默可抑制肿瘤生长，为靶向治疗提供依据。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“NAE1 antibody”“NEDD8激活酶”或“MLN4924”。
**Background of NAE1 Antibody**
NAE1 (NEDD8-Activating Enzyme E1), also known as APPBP1 or UBA3-interacting protein, is a critical subunit of the NEDD8-activating enzyme complex. This enzyme plays a central role in the NEDDylation pathway, a post-translational modification process analogous to ubiquitination. NAE1. in conjunction with UBA3. forms the heterodimeric E1 enzyme responsible for activating NEDD8 (Neural Precursor Cell Expressed Developmentally Downregulated Protein 8), a ubiquitin-like protein. The NAE1-UBA3 complex catalyzes ATP-dependent NEDD8 transfer to E2 conjugating enzymes, ultimately facilitating the attachment of NEDD8 to target proteins, such as cullin family members. This modification regulates the activity of cullin-RING ubiquitin ligases (CRLs), which are essential for protein degradation and cellular processes like cell cycle progression, DNA repair, and signal transduction.
NAE1 antibodies are valuable tools for studying the molecular mechanisms of NEDDylation and its implications in diseases, particularly cancer. Dysregulation of NAE1 and hyperactivation of NEDDylation are linked to tumorigenesis, making NAE1 a therapeutic target. Inhibitors like MLN4924 (Pevonedistat), which blocks NAE1 activity, are under clinical investigation. Researchers use NAE1 antibodies in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to assess protein expression, localization, and interaction partners in both normal and pathological contexts. These studies contribute to understanding cellular homeostasis, oncogenesis, and potential treatment strategies targeting the NEDD8 pathway.
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