| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CAII, Car2, CA-II |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRKAA2抗体的3篇模拟参考文献示例(内容基于已有研究主题概括，非真实文献)：
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1. **文献名称**: "AMPK α2 subunit deficiency affects cardiac metabolism and mitochondrial function in mice"
**作者**: Smith A, et al.
**摘要**: 本研究通过PRKAA2特异性抗体检测AMPK α2亚基在心脏组织中的表达，发现PRKAA2缺失导致小鼠心肌细胞能量代谢紊乱，线粒体功能受损，提示其在心脏代谢稳态中的关键作用。
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2. **文献名称**: "Role of AMPKα2 in regulating glucose uptake in skeletal muscle during exercise"
**作者**: Chen L, et al.
**摘要**: 利用PRKAA2抗体进行Western blot分析，作者揭示了运动诱导的AMPK α2激活促进骨骼肌葡萄糖摄取，其机制与GLUT4转运蛋白的调控相关。
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3. **文献名称**: "PRKAA2-mediated autophagy in cancer cell survival under metabolic stress"
**作者**: Wang Y, et al.
**摘要**: 该研究通过免疫组化(使用PRKAA2抗体)和基因沉默技术，证明AMPK α2在肿瘤细胞代谢应激下通过激活自噬途径维持细胞存活，为癌症靶向治疗提供新思路。
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**备注**：以上文献信息为示例性概括，实际引用请通过PubMed、Web of Science等数据库检索真实文献。建议使用关键词“PRKAA2 antibody”或“AMPK alpha2 antibody”结合研究主题(如代谢、疾病模型等)进行精确搜索。
The PRKAA2 antibody targets the protein encoded by the PRKAA2 gene, a critical subunit of AMP-activated protein kinase (AMPK). AMPK is a conserved energy-sensing enzyme that regulates cellular metabolism by maintaining energy homeostasis. It consists of three subunits: α (catalytic), β, and γ (regulatory). The PRKAA2 gene encodes the α2 subunit, which, along with the α1 subunit (PRKAA1), forms the kinase core responsible for AMPK's enzymatic activity. PRKAA2 is activated under low-energy conditions (e.g., ATP depletion, stress) and phosphorylates downstream targets to restore energy balance by inhibiting anabolic pathways and promoting catabolic processes like fatty acid oxidation and glucose uptake.
PRKAA2 is expressed in various tissues, including liver, skeletal muscle, and heart, with distinct roles in metabolic regulation compared to PRKAA1. Its activity is modulated by phosphorylation at Thr172 (within the kinase domain) and interactions with upstream kinases like LKB1 and CaMKKβ. Dysregulation of PRKAA2 has been linked to metabolic disorders (e.g., diabetes, obesity), cancer, and cardiovascular diseases, making it a therapeutic target.
PRKAA2 antibodies are essential tools for studying AMPK signaling. They enable detection of PRKAA2 expression, localization, and activation status via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specific antibodies can distinguish phosphorylated (active) or total PRKAA2. aiding research into metabolic pathways, drug development, and disease mechanisms. Validation of antibody specificity (e.g., using knockout models) is crucial to ensure accurate experimental outcomes.
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