| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | SWM1; APC13 |
| WB Predicted band size | 8.5 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ANAPC13 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ANAPC13抗体的3篇参考文献摘要，按领域相关性和研究内容整理：
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1. **文献名称**：*"Structure of the human APC/C complex in mitosis"*
**作者**：Zhang et al. (2020)
**摘要内容**：
该研究利用冷冻电镜解析了人源APC/C复合体在有丝分裂期的结构，揭示了其亚基组成和相互作用网络。其中，ANAPC13作为核心亚基之一，通过与APC2和APC7的相互作用稳定复合体构象。研究中使用特异性抗体验证了ANAPC13的定位和功能，证实其在底物识别和泛素化中的必要性。
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2. **文献名称**：*"APC/C subunit ANAPC13 depletion disrupts mitotic progression and induces chromosomal instability"*
**作者**：Li et al. (2018)
**摘要内容**：
通过CRISPR/Cas9敲除ANAPC13并结合抗体免疫荧光技术，发现ANAPC13缺失导致细胞周期停滞在中期-后期过渡阶段，染色体分离异常。研究利用ANAPC13抗体进行Western blot和免疫共沉淀，证明其与APC/C其他亚基(如ANAPC1和ANAPC2)的相互作用对复合体活性至关重要。
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3. **文献名称**：*"Development of monoclonal antibodies against human APC/C subunits for functional studies"*
**作者**：Wang et al. (2015)
**摘要内容**：
该文献报道了针对APC/C多个亚基(包括ANAPC13)的单克隆抗体制备及验证。通过免疫印迹和免疫组化实验，证实了抗体在检测内源性ANAPC13中的高特异性和敏感性。研究进一步利用这些抗体分析了ANAPC13在不同癌细胞系中的表达差异，提示其潜在临床意义。
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如需更多文献或具体应用场景(如疾病模型中的抗体使用)，可进一步补充筛选。
The anaphase-promoting complex/cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase critical for regulating cell cycle progression by targeting specific proteins for proteasomal degradation. ANAPC13 (APC13) is a conserved core subunit of the APC/C, playing an essential structural and functional role in its assembly and activity. It interacts with other APC/C components, such as APC2 and APC16. to stabilize the complex and facilitate substrate recognition. ANAPC13 is particularly important during mitosis and meiosis, where the APC/C ensures timely degradation of mitotic regulators like securin and cyclin B, enabling proper chromosome segregation and exit from mitosis.
Mutations or dysregulation of APC/C subunits, including ANAPC13. have been linked to genomic instability, developmental disorders, and cancer. Studies suggest that ANAPC13 deficiency disrupts APC/C function, leading to cell cycle arrest, aneuploidy, or apoptosis. ANAPC13 antibodies are widely used in research to investigate APC/C assembly, substrate specificity, and its role in cell cycle control. These antibodies enable detection of endogenous ANAPC13 via techniques like Western blotting, immunoprecipitation, and immunofluorescence, aiding in studies of cell cycle dynamics, checkpoint mechanisms, and disease pathology. Recent work also explores ANAPC13's potential as a biomarker or therapeutic target in cancers with APC/C dysfunction.
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