| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | OCTD |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human OTC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IRS1抗体的3篇参考文献示例(内容基于虚构摘要，仅供格式参考)：
1. **文献名称**：*"IRS-1 phosphorylation in insulin resistance and type 2 diabetes"*
**作者**：Sun, X.J., et al.
**摘要**：本研究利用IRS1特异性抗体检测了胰岛素抵抗患者肌肉组织中IRS1的酪氨酸磷酸化水平，发现其显著降低，提示磷酸化异常与2型糖尿病发病机制相关。
2. **文献名称**：*"Role of IRS1 in breast cancer progression via mTOR signaling"*
**作者**：Lee, J., & White, M.F.
**摘要**：通过免疫组化与Western blot分析，作者使用IRS1抗体证实了IRS1在乳腺癌细胞中高表达，并揭示了其通过mTOR通路促进肿瘤生长的分子机制。
3. **文献名称**：*"IRS1 knockout mice exhibit impaired glucose homeostasis"*
**作者**：Araki, E., et al.
**摘要**：研究利用IRS1抗体分析基因敲除小鼠的肝组织，发现IRS1缺失导致胰岛素信号传导缺陷，进一步验证了IRS1在糖代谢中的核心作用。
**注**：以上文献信息为模拟示例，实际引用请通过PubMed或学术数据库检索具体研究(关键词：IRS1 antibody、phosphorylation、insulin resistance、cancer)。
The insulin receptor substrate 1 (IRS1) antibody is a crucial tool in studying the IRS1 protein, a key mediator in insulin and insulin-like growth factor 1 (IGF-1) signaling pathways. IRS1. a cytoplasmic adaptor protein, facilitates signal transduction from activated insulin/IGF-1 receptors to downstream effectors like PI3K-AKT and MAPK, regulating glucose metabolism, cell growth, and survival. Dysregulation of IRS1 is linked to insulin resistance, type 2 diabetes, obesity, and cancers, making it a focal point in metabolic and oncological research.
IRS1 antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation to detect IRS1 expression, phosphorylation status, and interaction partners. These antibodies help elucidate IRS1's role in cellular responses under physiological and pathological conditions, including its post-translational modifications (e.g., serine/threonine phosphorylation) that modulate its activity.
Available as monoclonal or polyclonal forms, IRS1 antibodies vary in specificity and applications. Monoclonal antibodies offer consistency for quantitative assays, while polyclonal antibodies may detect multiple epitopes, enhancing sensitivity for low-abundance targets. Validation using IRS1 knockout models or siRNA knockdown is critical to confirm antibody specificity.
Commercially sourced from suppliers like Cell Signaling Technology, Abcam, and Santa Cruz Biotechnology, these antibodies drive advancements in understanding metabolic disorders and cancer mechanisms, aiding therapeutic target discovery.
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