| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human IRF8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于IRF8抗体的代表性文献(内容基于公开研究归纳，非真实文献)：
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1. **"IRF8 regulates myeloid cell development and autoimmune inflammation"**
*作者：Tamura T, et al. (2005)*
**摘要**：通过Western blot和流式细胞术分析IRF8抗体在小鼠髓系细胞中的表达，发现IRF8缺失导致树突状细胞分化缺陷，并加剧实验性自身免疫性脑脊髓炎(EAE)。
2. **"IRF8 expression in acute myeloid leukemia: a prognostic biomarker study"**
*作者：Schmidts A, et al. (2016)*
**摘要**：利用IRF8抗体进行免疫组织化学分析，发现IRF8低表达与急性髓系白血病(AML)患者的不良预后相关，提示其作为潜在治疗靶点。
3. **"IRF8-dependent tumor-associated macrophage polarization in colorectal cancer"**
*作者：AlMterfy M, et al. (2019)*
**摘要**：通过免疫荧光和流式细胞术结合IRF8抗体，揭示IRF8通过调控肿瘤相关巨噬细胞(TAMs)向抗肿瘤表型极化，抑制结直肠癌进展。
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如需具体文献，建议通过PubMed或Google Scholar以“IRF8 antibody”、“IRF8 immune function”等关键词检索。
The IRF8 antibody is a crucial tool for studying the interferon regulatory factor 8 (IRF8), a transcription factor belonging to the IRF family. IRF8. primarily expressed in hematopoietic cells, plays a pivotal role in immune cell differentiation and function, particularly in myeloid and lymphoid lineages. It regulates the development of macrophages, dendritic cells, and B cells, while also influencing T-cell responses. Dysregulation of IRF8 is linked to immune disorders, leukemias, and autoimmune diseases, making it a key focus in immunology and oncology research.
IRF8 antibodies are widely used in techniques like Western blotting, immunofluorescence, flow cytometry, and chromatin immunoprecipitation (ChIP) to detect IRF8 expression, localization, and DNA-binding activity. These antibodies aid in elucidating IRF8's role in immune signaling pathways, such as its interaction with PU.1 to regulate target genes, or its involvement in interferon-mediated responses. Monoclonal IRF8 antibodies offer high specificity, while polyclonal versions may detect multiple epitopes, enhancing sensitivity.
Validated IRF8 antibodies are critical for ensuring accurate experimental results, as cross-reactivity with other IRF family members (e.g., IRF4 or IRF5) can lead to misinterpretation. Researchers often verify antibody performance using knockout controls or siRNA-based silencing. Commercial IRF8 antibodies are typically tested for reactivity in human, mouse, and rat samples, supporting cross-species studies. Their applications span basic research into immune cell biology, cancer mechanisms, and therapeutic development, particularly in targeting IRF8-associated pathways for immunotherapy or precision medicine.
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