| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Clusterin alpha chain |
| Uniprot No | P10909 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-224aa |
| 氨基酸序列 | DQTVSDNELQEMSNQGSKYVNKEIQNAVNGVKQIKTLIEKTNEERKTLLSNLEEAKKKKEDALNETRESETKLKELPGVCNETMMALWEECKPCLKQTCMKFYARVCRSGSGLVGRQLEEFLNQSSPFYFWMNGDRIDSLLENDRQQTHMLDVMQDHFSRASSIIDELFQDRFFTREPQDTYHYLPFSLPHRRPHFFFPKSR |
| 预测分子量 | 27.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Clusterin alpha chain重组蛋白的3篇参考文献概览：
1. **文献名称**: "Recombinant Clusterin Alpha Chain Modulates Apoptosis in Prostate Cancer Cells"
**作者**: Smith, J.R., et al.
**摘要**: 研究重组Clusterin α链通过抑制Bax蛋白活化调控前列腺癌细胞凋亡的机制，证实其过表达可增强化疗耐药性。
2. **文献名称**: "Structural Characterization and Functional Analysis of Recombinant Human Clusterin Alpha Chain"
**作者**: Lee, H., & Kim, S.
**摘要**: 通过大肠杆菌表达系统成功纯化重组人Clusterin α链，并解析其晶体结构，揭示其与脂质结合能力及在神经退行性疾病中的潜在作用。
3. **文献名称**: "Expression and Purification of Biologically Active Clusterin Alpha Chain in a Mammalian System"
**作者**: Zhang, Y., et al.
**摘要**: 开发哺乳动物细胞表达体系生产具有糖基化修饰的功能性Clusterin α链，证实其抑制Aβ纤维形成的能力，为阿尔茨海默病治疗提供新策略。
注：上述文献信息为模拟示例，实际研究中建议通过PubMed或Web of Science以关键词“recombinant clusterin alpha chain”检索最新文献。部分研究可能聚焦于全长Clusterin，单独α链重组蛋白的专门研究相对较少。
Clusterin alpha chain recombinant protein is a biologically engineered form of the alpha subunit of clusterin, a multifunctional glycoprotein implicated in diverse cellular processes. Clusterin, also known as apolipoprotein J, exists as two primary isoforms: a secreted heterodimeric protein (sCLU) and a nuclear form (nCLU). The secreted isoform, produced through alternative splicing, consists of alpha and beta chains linked by disulfide bonds. It functions as a molecular chaperone, stabilizing stressed proteins, modulating apoptosis, and regulating inflammation, complement activity, and lipid transport.
The alpha chain, encoded by the *CLU* gene, contains critical domains for protein-protein interactions and cellular signaling. Recombinant clusterin alpha chain is typically produced using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. This allows researchers to study its structural and functional roles independently or in combination with the beta chain.
Clusterin's dual roles in both cytoprotection and pathology make it a focus in disease research. Elevated sCLU levels are associated with cancer progression, neurodegenerative disorders (e.g., Alzheimer’s), atherosclerosis, and aging. Recombinant alpha chain proteins are used to investigate its anti-apoptotic effects, interactions with pathogenic aggregates (e.g., amyloid-beta), and modulation of cell survival pathways like PI3K/Akt and NF-κB. Additionally, they serve as tools to develop therapeutic strategies, such as blocking clusterin in chemotherapy-resistant cancers or enhancing its neuroprotective functions.
Despite its potential, challenges remain in understanding isoform-specific mechanisms and reconciling contradictory roles in different tissues. Recombinant alpha chain studies aim to clarify these complexities, offering insights into targeted therapies and diagnostic biomarkers.
×
