| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX19B |
| Uniprot No | Q9UMR2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-479aa |
| 氨基酸序列 | ATDSWALAVDEQEAAAESLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQSLLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQENALPLMLAEPPQNLIAQSQSGTGKTAAFVLAMLSQVEPANKYPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN |
| 预测分子量 | 80.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DDX19B重组蛋白的模拟参考文献示例，基于相关研究领域的典型方向整理而成：
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1. **"Structural and functional analysis of DDX19B in mRNA export"**
*作者：Strässer, K., et al.*
**摘要**：本研究通过重组DDX19B蛋白解析了其在mRNA出核转运中的构象变化，揭示了ATP酶活性对RNA结合的调控机制，并利用体外重组系统验证了其与核孔蛋白Nup214的互作。
2. **"DDX19B interacts with HIV-1 Rev to facilitate viral RNA export"**
*作者：Fischer, U., et al.*
**摘要**：文章利用重组DDX19B蛋白进行互作实验，发现其通过结合HIV-1 Rev蛋白促进病毒RNA的核输出，为抗病毒靶点开发提供了理论依据。
3. **"Overexpression of DDX19B promotes tumor metastasis via Wnt/β-catenin signaling"**
*作者：Sekimoto, T., et al.*
**摘要**：通过重组DDX19B表达模型，作者证明其在结肠癌细胞中异常激活Wnt通路，并揭示了其C端结构域对下游信号转导的关键作用。
4. **"Crystal structure of DDX19B reveals RNA-binding motifs and ATP-dependent conformational changes"**
*作者：Montpetit, B., et al.*
**摘要**：该研究解析了重组DDX19B蛋白的晶体结构，阐明了其DEAD-box核心域与RNA结合的分子基础，并提出了ATP水解驱动功能循环的结构模型。
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*注：以上文献为示例性模拟，实际引用时需以真实发表的论文为准。建议通过PubMed或Google Scholar检索“DDX19B recombinant protein”获取准确信息。*
DDX19B, a member of the DEAD-box RNA helicase family, plays a critical role in mRNA export and translation regulation. This ATP-dependent RNA helicase is primarily localized at the cytoplasmic side of nuclear pore complexes (NPCs), where it facilitates the release of mRNA transcripts into the cytoplasm during nucleocytoplasmic transport. Structurally characterized by conserved helicase core domains, DDX19B interacts with key components of the mRNA export machinery, including Nup214 and Gle1. while its activity is modulated by phosphorylation and ATP/RNA binding.
Recombinant DDX19B proteins are engineered to study its molecular mechanisms, often expressed in bacterial or insect cell systems with affinity tags (e.g., His-tag) for purification. These tools enable investigations into its helicase activity, RNA-binding specificity, and interactions with transport factors. Studies using recombinant DDX19B have revealed its dual role in mRNA export and translation termination, particularly in resolving secondary structures of nascent transcripts and recycling ribosomal subunits.
Dysregulation of DDX19B has been linked to viral infections, neurodegenerative diseases, and cancers, making it a potential therapeutic target. Recombinant forms are essential for high-throughput drug screening and structural analyses (e.g., cryo-EM) to visualize its conformational changes during ATP hydrolysis. Recent research also highlights its involvement in cellular stress responses and mitotic progression, underscoring its multifaceted functions beyond canonical mRNA transport. The development of mutant variants (e.g., ATPase-deficient forms) further aids in dissecting its mechanistic contributions to gene expression regulation.
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