| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC19A3 |
| Uniprot No | Q9BZV2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496aa |
| 氨基酸序列 | MDCYRTSLSSSWIYPTVILCLFGFFSMMRPSEPFLIPYLSGPDKNLTSAEITNEIFPVWTYSYLVLLLPVFVLTDYVRYKPVIILQGISFIITWLLLLFGQGVKTMQVVEFFYGMVTAAEVAYYAYIYSVVSPEHYQRVSGYCRSVTLAAYTAGSVLAQLLVSLANMSYFYLNVISLASVSVAFLFSLFLPMPKKSMFFHAKPSREIKKSSSVNPVLEETHEGEAPGCEEQKPTSEILSTSGKLNKGQLNSLKPSNVTVDVFVQWFQDLKECYSSKRLFYWSLWWAFATAGFNQVLNYVQILWDYKAPSQDSSIYNGAVEAIATFGGAVAAFAVGYVKVNWDLLGELALVVFSVVNAGSLFLMHYTANIWACYAGYLIFKSSYMLLITIAVFQIAVNLNVERYALVFGINTFIALVIQTIMTVIVVDQRGLNLPVSIQFLVYGSYFAVIAGIFLMRSMYITYSTKSQKDVQSPAPSENPDVSHPEEESNIIMSTKL |
| 预测分子量 | 55kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC19A3重组蛋白的模拟参考文献示例(仅供格式参考，非真实文献)：
1. **"Functional characterization of recombinant human SLC19A3 in cellular thiamine transport"**
*作者：Zeng W, et al.*
**摘要**：研究通过HEK293细胞表达重组SLC19A3蛋白，证实其介导硫胺素(维生素B1)的高亲和力转运，并发现其活性受pH和突变影响，为相关代谢疾病机制提供依据。
2. **"Structural insights into SLC19A3 dynamics by cryo-EM analysis"**
*作者：Subramanian S, et al.*
**摘要**：利用冷冻电镜解析重组人源SLC19A3蛋白的三维结构，揭示其跨膜结构域中硫胺素结合的关键氨基酸残基，阐明转运过程中的构象变化。
3. **"Recombinant SLC19A3 rescues thiamine deficiency in a murine model"**
*作者：Kumar A, et al.*
**摘要**：在小鼠模型中，通过腺病毒递送重组SLC19A3蛋白，成功逆转因基因突变导致的硫胺素缺乏症，验证其潜在治疗应用价值。
4. **"Expression and purification of SLC19A3 in prokaryotic systems"**
*作者：Diaz R, et al.*
**摘要**：报道在大肠杆菌中高效表达可溶性重组SLC19A3蛋白的优化方案，并通过体外实验验证其结合硫胺素的生物活性。
注：以上为模拟文献，实际引用时需查询真实数据库(如PubMed)获取准确信息。
The solute carrier family 19 member 3 (SLC19A3) protein is a transmembrane transporter primarily involved in thiamine (vitamin B1) uptake, essential for cellular energy metabolism and neurological function. Encoded by the SLC19A3 gene, this protein belongs to the SLC19 family, which includes transporters with roles in folate and thiamine homeostasis. SLC19A3 is highly expressed in the brain, liver, and intestines, where it facilitates thiamine transport across cell membranes via pH-dependent mechanisms. Its dysfunction is linked to severe genetic disorders such as thiamine metabolism dysfunction syndrome 2 (THMD2), biotin-thiamine-responsive basal ganglia disease, and Leigh syndrome, characterized by neurodegeneration and metabolic crises.
Recombinant SLC19A3 protein is produced using biotechnological platforms (e.g., mammalian or insect cell systems) to express the purified, functional protein for research and therapeutic development. This engineered protein retains key structural features, including 12 predicted transmembrane domains and critical substrate-binding regions, enabling studies on transport kinetics, substrate specificity, and molecular interactions. Researchers use recombinant SLC19A3 to investigate disease mechanisms, screen potential therapeutics (e.g., thiamine analogs or chaperone molecules), and develop gene therapy vectors. Its applications extend to structural biology (e.g., cryo-EM studies) to resolve conformational changes during thiamine transport. By elucidating SLC19A3’s role in cellular thiamine homeostasis, recombinant variants contribute to advancing treatments for rare metabolic disorders and optimizing nutritional uptake pathways.
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