| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCTL |
| Uniprot No | Q6UWM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-541aa |
| 氨基酸序列 | RKGSPEEASFYYGTFPLGFSWGVGSSAYQTEGAWDQDGKGPSIWDVFTHSGKGKVLGNETADVACDGYYKVQEDIILLRELHVNHYRFSLSWPRLLPTGIRAEQVNKKGIEFYSDLIDALLSSNITPIVTLHHWDLPQLLQVKYGGWQNVSMANYFRDYANLCFEAFGDRVKHWITFSDPRAMAEKGYETGHHAPGLKLRGTGLYKAAHHIIKAHAKAWHSYNTTWRSKQQGLVGISLNCDWGEPVDISNPKDLEAAERYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSLQEKSYIKGTSDFLGLGHFTTRYITERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYSVPWGFRRLLNFAQTQYGDPPIYVMENGASQKFHCTQLCDEWRIQYLKGYINEMLKAIKDGANIKGYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKASVQYYKKIIIANGFPNPREVESWYLKALETCSINNQMLAAEPLLSHMQMVTE |
| 预测分子量 | 67.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LCTL重组蛋白的3篇参考文献示例(注：内容为虚构示例，仅用于演示格式)：
1. **文献名称**: *Cloning and Functional Characterization of Recombinant LCTL in E. coli*
**作者**: Smith J, et al.
**摘要**: 本研究成功将LCTL基因克隆至大肠杆菌表达系统，优化诱导条件后获得高纯度可溶性蛋白，并证实其具有长链脂肪酸硫酯酶活性，为后续酶学分析奠定基础。
2. **文献名称**: *Structural Insights into LCTL via X-ray Crystallography*
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析LCTL重组蛋白的三维结构，揭示其底物结合口袋的关键氨基酸残基，为设计特异性抑制剂提供结构基础。
3. **文献名称**: *LCTL Recombinant Protein in Lipid Metabolism: In vivo Functional Study*
**作者**: Garcia R, et al.
**摘要**: 在小鼠模型中过表达LCTL重组蛋白，发现其显著影响肝脏脂质代谢，降低甘油三酯积累，提示其在代谢疾病治疗中的潜在应用价值。
(如需真实文献，建议在PubMed或Web of Science中检索关键词“LCTL recombinant protein”或联系领域专家获取。)
**Background of LCTL Recombinant Protein**
LCTL (Long-Chain Fatty Acid Transport Protein 1), also known as SLC27A1. is a membrane-associated protein involved in cellular lipid metabolism. It facilitates the uptake of long-chain fatty acids (LCFAs) by acting as a transporter and acyl-CoA synthetase, converting extracellular LCFAs into acyl-CoA esters for subsequent metabolic processes. This dual functionality links LCTL to energy homeostasis, membrane synthesis, and signaling pathways, making it critical in tissues with high metabolic demands, such as the liver, heart, and adipose tissue.
Recombinant LCTL protein is engineered using biotechnological platforms (e.g., bacterial, insect, or mammalian expression systems) to produce purified, functional forms of the protein for research and therapeutic applications. Its recombinant form retains enzymatic and transport activities, enabling studies on lipid metabolism dysregulation in diseases like obesity, diabetes, and cardiovascular disorders. Structural analysis of recombinant LCTL has provided insights into its substrate-binding domains and catalytic mechanisms, aiding drug discovery efforts targeting metabolic syndromes.
Recent studies highlight LCTL's role in cancer progression, as altered fatty acid metabolism is a hallmark of tumor cells. Recombinant LCTL serves as a tool to investigate tumor lipid dependencies and evaluate inhibitors that disrupt fatty acid uptake in oncology research. Additionally, it supports diagnostic assay development for metabolic disorders and serves as an antigen in antibody production.
Despite progress, challenges remain in optimizing recombinant LCTL stability and activity *in vitro*. Ongoing research focuses on structural engineering, post-translational modification mimicry, and hybrid expression systems to enhance its utility. As lipid metabolism gains attention in precision medicine, recombinant LCTL remains a pivotal reagent for elucidating disease mechanisms and advancing therapeutic strategies.
×
