| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC44A4 |
| Uniprot No | Q53GD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 56-229aa |
| 氨基酸序列 | WLYGDPRQVLYPRNSTGAYCGMGENKDKPYLLYFNIFSCILSSNIISVAENGLQCPTPQVCVSSCPEDPWTVGKNEFSQTVGEVFYTKNRNFCLPGVPWNMTVITSLQQELCPSFLLPSAPALGRCFPWTNVTPPALPGITNDTTIQQGISGLIDSLNARDISVKIFEDFAQSW |
| 预测分子量 | 26.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC44A4重组蛋白的3篇参考文献示例(注：文献为虚拟概括，实际研究中可能需要进一步检索验证)：
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1. **标题**: *"Expression and Functional Characterization of Recombinant SLC44A4 as a Choline Transporter in Mammalian Cells"*
**作者**: Smith A, et al.
**摘要**: 研究报道了SLC44A4重组蛋白在HEK293细胞中的表达与纯化，证实其具有胆碱转运活性，并分析了pH依赖性对转运效率的影响。
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2. **标题**: *"Structural Insights into SLC44A4 Through Recombinant Protein Crystallography"*
**作者**: Chen L, et al.
**摘要**: 通过重组SLC44A4蛋白的晶体结构解析，揭示了其跨膜结构域的关键氨基酸残基，为设计靶向该蛋白的小分子抑制剂提供依据。
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3. **标题**: *"SLC44A4 Recombinant Protein as a Novel Biomarker in Colorectal Cancer Serum Detection"*
**作者**: Wang Y, et al.
**摘要**: 利用重组SLC44A4蛋白开发ELISA检测方法，发现其在结直肠癌患者血清中高表达，提示其作为诊断标志物的潜力。
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(注：若需真实文献，建议在PubMed或SciFinder中以“SLC44A4 recombinant”为关键词检索。)
SLC44A4 (Solute Carrier Family 44 Member 4) is a transmembrane protein belonging to the SLC44 family, which is implicated in the transport of choline and other organic cations. This protein is encoded by the *SLC44A4* gene and is primarily expressed in epithelial tissues, including the colon, pancreas, and prostate. Its physiological role involves modulating cellular uptake of choline, a critical precursor for acetylcholine synthesis and phospholipid metabolism, which is essential for maintaining membrane integrity and signaling processes. Dysregulation of SLC44A4 has been associated with various pathologies, including colorectal cancer, where its overexpression is observed in tumor tissues, suggesting potential roles in oncogenesis or cancer progression.
Recombinant SLC44A4 protein is engineered through molecular cloning techniques, often expressed in heterologous systems like mammalian cells or bacteria to ensure proper folding and post-translational modifications. The recombinant form enables detailed functional studies, such as substrate specificity, transport kinetics, and interaction partners, which are challenging to explore in native cellular environments. Structurally, the protein typically retains key domains, such as the 10 transmembrane helices characteristic of SLC44 family members, facilitating choline-binding and ion translocation.
Research on SLC44A4 recombinant protein has expanded into diagnostic and therapeutic applications. For instance, it serves as an antigen for developing antibodies used in immunohistochemistry to detect cancer biomarkers. Additionally, its role in choline metabolism links it to metabolic disorders and neurological conditions, prompting investigations into pharmacological modulators. Despite progress, the full mechanistic understanding of SLC44A4 remains incomplete, necessitating further studies to clarify its regulatory networks and potential as a therapeutic target in precision medicine.
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