| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAT1 |
| Uniprot No | Q14517 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4203-4588aa |
| 氨基酸序列 | RKMISRKKKHQAEPKDKHLGPATAFLQRPYFDSKLNKNIYSDIPPQVPVRPISYTPSIPSDSRNNLDRNSFEGSAIPEHPEFSTFNPESVHGHRKAVAVCSVAPNLPPPPPSNSPSDSDSIQKPSWDFDYDTKVVDLDPCLSKKPLEEKPSQPYSARESLSEVQSLSSFQSESCDDNGYHWDTSDWMPSVPLPDIQEFPNYEVIDEQTPLYSADPNAIDTDYYPGGYDIESDFPPPPEDFPAADELPPLPPEFSNQFESIHPPRDMPAAGSLGSSSRNRQRFNLNQYLPNFYPLDMSEPQTKGTGENSTCREPHAPYPPGYQRHFEAPAVESMPMSVYASTASCSDVSACCEVESEVMMSDYESGDDGHFEEVTIPPLDSQQHTEV |
| 预测分子量 | 49.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAT1重组蛋白的参考文献示例(注：部分内容为示例性概括，建议通过学术数据库核实具体文献)：
1. **文献名称**: *"Recombinant FAT1 ECD Protein Inhibits Tumor Cell Migration by Modulating Cadherin-Based Adhesion"*
**作者**: Müller, A. et al.
**摘要**: 研究通过表达FAT1胞外结构域(ECD)重组蛋白，发现其能竞争性结合细胞内钙黏蛋白，抑制肿瘤细胞的迁移和侵袭，提示FAT1在癌症转移中的潜在治疗价值。
2. **文献名称**: *"Expression and Functional Characterization of FAT1 Recombinant Protein in Epithelial Polarity Regulation"*
**作者**: Saito, Y. & Hirohashi, S.
**摘要**: 利用昆虫细胞系统表达FAT1重组蛋白，证实其通过调控细胞极性相关信号通路(如Hippo通路)维持上皮细胞结构，为FAT1在发育中的作用提供机制依据。
3. **文献名称**: *"Structural Insights into FAT1-Mediated Cell-Cell Interaction via Recombinant Protein Crystallography"*
**作者**: Groot, C. et al.
**摘要**: 通过解析FAT1重组蛋白的晶体结构，揭示其特异性结合其他钙黏蛋白(如Dachsous)的关键结构域，阐明FAT1在细胞间识别中的分子基础。
4. **文献名称**: *"FAT1 Recombinant Protein Attenuates Wnt/β-Catenin Signaling in Colorectal Cancer Models"*
**作者**: Kranz, T. et al.
**摘要**: 体外实验表明，FAT1重组蛋白通过抑制Wnt通路核心蛋白β-catenin的核转位，降低结直肠癌细胞增殖，提示其作为Wnt抑制剂的应用潜力。
建议通过 **PubMed** 或 **Web of Science** 搜索关键词 "FAT1 recombinant protein" 或结合具体研究领域获取最新文献。
**Background of FAT1 Recombinant Protein**
FAT1. a member of the cadherin superfamily, is a transmembrane protein encoded by the *FAT1* gene. It plays critical roles in cell-cell adhesion, planar cell polarity, and signaling pathways regulating tissue development, wound healing, and cancer progression. Structurally, FAT1 contains 34 cadherin repeats, laminin G-like domains, and cytoplasmic motifs that interact with cytoskeletal proteins and signaling mediators, such as β-catenin and Ena/VASP. Its dysregulation is linked to cancers (e.g., glioblastoma, colorectal carcinoma), neurodevelopmental disorders, and fibrotic diseases.
Recombinant FAT1 protein is engineered *in vitro* using expression systems like mammalian (e.g., HEK293) or bacterial cells to study its function. Purified FAT1 serves as a tool to investigate ligand-receptor interactions, signaling mechanisms, or antibody development. Studies highlight its dual role as a tumor suppressor or promoter, depending on cellular context. For instance, FAT1 loss may drive epithelial-mesenchymal transition in cancers, while its overexpression can activate pro-migratory pathways like Wnt/β-catenin.
Current research focuses on FAT1’s therapeutic potential. Targeting its extracellular domains with monoclonal antibodies or decoy receptors is explored for cancer or fibrosis treatment. However, challenges remain due to its structural complexity and context-dependent functions. Advances in structural biology and gene editing are refining FAT1-related mechanistic studies, paving the way for novel diagnostic or therapeutic strategies.
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