| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRE2 |
| Uniprot No | Q12068 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342aa |
| 氨基酸序列 | MSVFVSGANGFIAQHIVDLLLKEDYKVIGSARSQEKAENLTEAFGNNPKFSMEVVPDISKLDAFDHVFQKHGKDIKIVLHTASPFCFDITDSERDLLIPAVNGVKGILHSIKKYAADSVERVVLTSSYAAVFDMAKENDKSLTFNEESWNPATWESCQSDPVNAYCGSKKFAEKAAWEFLEENRDSVKFELTAVNPVYVFGPQMFDKDVKKHLNTSCELVNSLMHLSPEDKIPELFGGYIDVRDVAKAHLVAFQKRETIGQRLIVSEARFTMQDVLDILNEDFPVLKGNIPVGKPGSGATHNTLGATLDNKKSKKLLGFKFRNLKETIDDTASQILKFEGRI |
| 预测分子量 | 44.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GRE2重组蛋白的3篇参考文献示例，包含文献名称、作者及摘要内容概括：
1. **文献名称**: *Heterologous Expression and Characterization of GRE2 Aldo-Keto Reductase in Escherichia coli*
**作者**: Smith J, Brown K, Lee T.
**摘要**: 该研究在大肠杆菌中成功异源表达了来自酿酒酵母的GRE2醛酮还原酶，通过亲和层析纯化重组蛋白，并测定其对多种醛类底物(如甲基乙二醛和丙酮醛)的催化活性，揭示了其NADPH依赖的还原酶特性及在氧化应激中的潜在作用。
2. **文献名称**: *Enhancing Osmotic Stress Tolerance in Yeast by Overexpression of Recombinant GRE2*
**作者**: Zhang Y, Wang L, Chen H.
**摘要**: 通过构建重组GRE2过表达酵母菌株，研究发现其在渗透压胁迫(高盐、高糖)下的存活率显著提升，表明GRE2通过调控甘油合成途径增强细胞适应性，为工业酵母工程改造提供了新策略。
3. **文献名称**: *Structural and Functional Analysis of Recombinant GRE2: Insights into Substrate Specificity*
**作者**: Müller S, García-Ruiz E, Alcalde M.
**摘要**: 本研究解析了重组GRE2蛋白的晶体结构，结合分子对接实验，鉴定了底物结合口袋的关键氨基酸残基，揭示了其对不同醛类底物的选择性机制，为酶工程优化提供了结构基础。
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**备注**：上述文献信息为示例性质，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议使用关键词“GRE2 recombinant protein”或“GRE2 aldo-keto reductase”结合具体研究方向进一步筛选。
**Background of GRE2 Recombinant Protein**
GRE2. also known as **NADPH-dependent methylglyoxal reductase**, is a yeast enzyme encoded by the *GRE2* gene in *Saccharomyces cerevisiae*. It plays a dual role in cellular detoxification and stress response, primarily by reducing toxic aldehydes and ketones generated during metabolic processes. GRE2 is notably involved in the glutathione-independent detoxification pathway, converting harmful compounds like methylglyoxal (a reactive glycolytic byproduct) into less toxic alcohols, thereby protecting cells from oxidative and carbonyl stress. Its expression is upregulated under various stress conditions, including osmotic stress, xenobiotic exposure, and nutrient limitations, mediated by stress-responsive transcription factors such as Msn2/4.
The **recombinant GRE2 protein** is engineered through heterologous expression systems, such as *E. coli* or yeast, to produce purified enzyme for functional and structural studies. Recombinant technology allows scalable production, enabling detailed investigation of its substrate specificity, catalytic mechanisms, and potential biotechnological applications. GRE2’s broad substrate spectrum and stereoselectivity make it valuable in synthetic biology for producing chiral alcohols, pharmaceutical intermediates, or biofuels. Additionally, studies on GRE2 contribute to understanding eukaryotic stress adaptation mechanisms, offering insights into human diseases linked to oxidative stress or carbonyl overload, such as diabetes and neurodegenerative disorders. Recent research also explores its role in metabolic engineering to enhance microbial resistance to industrial fermentation stresses, underscoring its industrial relevance.
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