| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | pknF |
| Uniprot No | P9WI74 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476aa |
| 氨基酸序列 | MPLAEGSTFAGFTIVRQLGSGGMGEVYLARHPRLPRQDALKVLRADVSADGEYRARFNREADAAASLWHPHIVAVHDRGEFDGQLWIDMDFVDGTDTVSLLRDRYPNGMPGPEVTEIITAVAEALDYAHERRLLHRDVKPANILIANPDSPDRRIMLADFGIAGWVDDPSGLTATNMTVGTVSYAAPEQLMGNELDGRADQYALAATAFHLLTGSPPFQHANPAVVISQHLSASPPAIGDRVPELTPLDPVFAKALAKQPKDRYQRCVDFARALGHRLGGAGDPDDTRVSQPVAVAAPAKRSLLRTAVIVPAVLAMLLVMAVAVAVREFQRADDERAAQPARTRTTTSAGTTTSVAPASTTRPAPTTPTTTGAADTATASPTAAVVAIGALCFPLGSTGTTKTGATAYCSTLQGTNTTIWSLTEDTVASPTVTATADPTEAPLPIEQESPIRVCMQQTGQTRRECREEIRRSNGWP |
| 预测分子量 | 78.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"The Ser/Thr protein kinase PknF of Mycobacterium tuberculosis"**
- 作者：Molle, V. et al.
- 摘要：研究结核杆菌中PknF的激酶活性及自磷酸化作用，揭示其在调控细胞分裂和应激反应中的潜在功能。
2. **"Crystal structure of the catalytic domain of PknF from Mycobacterium tuberculosis"**
- 作者：Greenstein, A.E. et al.
- 摘要：解析PknF激酶结构域的三维晶体结构，揭示其ATP结合位点特征，为靶向药物设计提供结构基础。
3. **"Functional characterization of the PknF recombinant protein in mycobacterial survival"**
- 作者：Kang, C.-M. et al.
- 摘要：通过重组蛋白实验证明PknF在结核杆菌缺氧胁迫下的存活中起关键作用，可能与能量代谢调控相关。
4. **"Recombinant PknF kinase: a tool for studying mycobacterial signal transduction"**
- 作者：Fernandez, P. et al.
- 摘要：开发重组PknF蛋白体外活性分析体系，揭示其与下游转录因子相互作用，参与病原体毒力调控的分子机制。
**Background of pknF Recombinant Protein**
The pknF recombinant protein originates from *Mycobacterium tuberculosis* (Mtb), the pathogenic bacterium responsible for tuberculosis (TB). PknF is a member of the eukaryotic-like serine/threonine protein kinase (STPK) family, which plays a critical role in regulating bacterial growth, stress responses, and virulence. Unlike most prokaryotes, Mtb employs STPKs for signal transduction, mimicking eukaryotic regulatory mechanisms. PknF, encoded by the *pknF* gene, is a transmembrane kinase characterized by an N-terminal kinase domain and a C-terminal regulatory region. It undergoes autophosphorylation to activate downstream signaling pathways, influencing processes like cell wall synthesis, metabolic adaptation, and survival under hostile host environments.
Recombinant pknF is produced through genetic engineering, typically by cloning the *pknF* gene into expression vectors (e.g., *E. coli*), followed by purification using affinity chromatography. This engineered protein retains the functional kinase activity of native pknF, enabling in vitro studies to dissect its biochemical properties, substrate interactions, and regulatory mechanisms. Researchers utilize pknF recombinant protein to investigate its role in Mtb pathogenesis, particularly in mediating resistance to oxidative stress and antibiotics.
Studying pknF is crucial for understanding TB pathophysiology and drug development. As multidrug-resistant TB strains emerge, targeting bacterial signaling pathways offers a promising therapeutic strategy. Inhibitors of pknF could disrupt Mtb’s ability to adapt and survive, potentially enhancing antibiotic efficacy. Additionally, structural analyses of recombinant pknF provide insights into kinase activation and substrate binding, guiding the design of novel antimycobacterial agents. Thus, pknF recombinant protein serves as a vital tool in both basic research and applied biomedical efforts to combat tuberculosis.
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