| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LF2 |
| Uniprot No | P0C725 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-429aa |
| 氨基酸序列 | MAEAYPGGAHAALASRRSSFRNSLRRLRPTEKPDTSFMRGVWKYEIFPSYVRVTNKQVLQLDAQCQELPPCPSVGQILSFKLPSFSFNTTTYGSRYFTVAFLFFGAEDNEVFLKPFFVMHSDQDIVLSVLNPRSLFIEKGKFTWYIVPIRLVKNPYLYLQILPGQSDIQLTRSCTQSGDKLNTSEPQIFLSGSPVTSQDECLPYLLAQHTPPFLKSYARIHTFPGKVCPVNAIRRGKGYVRVSVDTPDLKREGPLNVKVGMTLLDDVIIAFRYNPYPKSHWRWDGESTDIRYFGSPVIIPPNFITELEYNNTYEAPLSSKITAVVVSHSSNPVFYVYPQEWKPGQTLKLTVRNISNNPITIVTGQSMAQAFFIYAGDPSISTIMRRYIQRQGCALTLPGNIVVESSSLPTFERINKTFNGNIVASEGTL |
| 预测分子量 | 53.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LF2重组蛋白的模拟参考文献示例(文献信息为虚构，仅供格式参考)：
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1. **文献名称**：*LF2 Recombinant Protein Suppresses c-Myc Activity in Burkitt Lymphoma Cells*
**作者**：Schuhmacher, M. et al.
**摘要**：研究利用重组LF2蛋白调控c-Myc致癌基因活性，发现其通过结合c-Myc转录复合物抑制肿瘤细胞增殖，为淋巴瘤治疗提供潜在靶点。
2. **文献名称**：*Structural Characterization of Anthrax Lethal Factor (LF2) and Its Interaction with Substrates*
**作者**：Vitale, G. et al.
**摘要**：通过重组表达炭疽致死因子LF2.解析其三维结构及催化机制，揭示LF2与底物结合的关键氨基酸位点，为抑制剂设计奠定基础。
3. **文献名称**：*LF2 Recombinant Protein Induces Apoptosis in Hepatocellular Carcinoma via p53 Pathway*
**作者**：Chen, L. et al.
**摘要**：实验证明重组LF2蛋白通过激活p53依赖性凋亡通路抑制肝癌细胞生长，体内实验显示显著降低肿瘤体积。
4. **文献名称**：*LF2 as a Novel Immune Checkpoint Regulator: Recombinant Protein Enhances T Cell Activation*
**作者**：Zhang, Y. et al.
**摘要**：首次报道重组LF2蛋白通过调控PD-1/PD-L1信号轴增强T细胞抗肿瘤活性，提出其在免疫治疗中的应用潜力。
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**注**：以上文献为示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
**Background of LF2 Recombinant Protein**
LF2 recombinant protein is a biologically engineered derivative of the lethal factor 2 (LF2), a critical component of the anthrax toxin produced by *Bacillus anthracis*. Anthrax toxin comprises three subunits: protective antigen (PA), edema factor (EF), and lethal factor (LF). LF2. a truncated or modified variant of LF, retains functional domains essential for its role in pathogenesis. Structurally, LF2 is a zinc-dependent metalloprotease that targets mitogen-activated protein kinase kinases (MAPKKs), disrupting intracellular signaling pathways and inducing cell death.
Recombinant LF2 is produced using expression systems such as *E. coli* or mammalian cells, enabling scalable and controlled synthesis. The gene encoding LF2 is cloned into a plasmid vector, expressed in host cells, and purified via chromatography. This recombinant approach ensures high purity and consistency, critical for research and therapeutic applications.
LF2 recombinant protein is widely utilized in studying anthrax toxin mechanisms, including host-pathogen interactions and cellular uptake processes mediated by PA. It also serves as a tool for screening inhibitors or antibodies that neutralize its protease activity, aiding in the development of anthrax therapeutics. Additionally, LF2 has been explored in targeted drug delivery systems, leveraging its ability to translocate cargo into cells when fused with other proteins.
Safety considerations are paramount due to LF2’s inherent toxicity. Researchers employ inactivated forms or stringent containment protocols during handling. Beyond anthrax research, LF2’s unique properties contribute to broader studies in protein engineering, immunology, and cellular signaling, underscoring its versatility in biomedical applications.
In summary, LF2 recombinant protein bridges fundamental research and translational innovation, offering insights into bacterial pathogenesis while holding potential for novel therapies and biotechnological tools.
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