| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | bcsP31 |
| Uniprot No | P0A3T2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-329aa |
| 氨基酸序列 | QAPTFFRIGTGGTAGTYYPIGGLIANAISGAGEKGVPGLVATAVSSNGSVANINAIKSGALESGFTQSDVAYWAYNGTGLYDGKGKVEDLRLLATLYPETIHIVARKDANIKSVADLKGKRVSLDEPGSGTIVDARIVLEAYGLTEDDIKAEHLKPGPAGERLKDGALDAYFFVGGYPTGAISELAISNGISLVPISGPEADKILEKYSFFSKDVVPAGAYKDVAETPTLAVAAQWVTSAKQPDDLIYNITKVLWNEDTRKALDAGHAKGKLIKLDSATSSLGIPLHPGAERFYKEAGVLK |
| 预测分子量 | 39.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BcsP31重组蛋白的3篇参考文献概览：
---
1. **文献名称**: *Heterologous Expression and Functional Analysis of the BcsP31 Protein in Bacterial Cellulose Biosynthesis*
**作者**: Smith J, et al.
**摘要**: 研究在大肠杆菌中重组表达BcsP31蛋白，证实其与纤维素合成酶复合体互作，通过体外酶活实验揭示其调控纤维素聚合的关键作用。
---
2. **文献名称**: *Structural Characterization of Recombinant BcsP31 from *Komagataeibacter xylinus* and Its Role in Biofilm Formation*
**作者**: Lee H, Kim S.
**摘要**: 报道了BcsP31重组蛋白的晶体结构解析，发现其C端结构域参与细菌生物膜基质的组装，为靶向治疗提供新思路。
---
3. **文献名称**: *Cloning and Purification of BcsP31: Implications for Synthetic Biology Applications*
**作者**: García R, et al.
**摘要**: 开发了高效可溶表达BcsP31的毕赤酵母系统，证明重组蛋白可整合人工纤维素材料合成途径，推动生物制造应用。
---
注：以上为模拟文献，实际研究中建议通过**PubMed/Google Scholar**以关键词“BcsP31 recombinant protein”或“BcsP31 cellulose synthase”检索最新实证论文。
The BcsP31 recombinant protein is derived from the Bcs (bacterial cellulose synthase) complex, a multi-enzyme system primarily studied in *Komagataeibacter* species (formerly *Gluconacetobacter*), which are known for producing high-purity bacterial cellulose. The Bcs complex consists of multiple subunits (BcsA, BcsB, BcsC, BcsD, etc.) that collectively catalyze cellulose biosynthesis. BcsP31. a 31 kDa accessory protein encoded within the bacterial cellulose synthase operon, plays a regulatory role in cellulose production, though its exact molecular mechanism remains under investigation. Studies suggest it may interact with other Bcs components to modulate cellulose crystallinity, chain elongation, or extracellular export.
Recombinant BcsP31 is engineered for heterologous expression, typically in *E. coli* systems, to enable functional and structural studies. Its recombinant production allows purification of soluble protein for biochemical assays, crystallization trials, and interaction analyses. Researchers utilize BcsP31 to explore bacterial cellulose biosynthesis pathways, aiming to optimize industrial cellulose production for applications in biomaterials, medical implants, and sustainable textiles. Interest in this protein has grown alongside the demand for bacterial cellulose in wound dressings, drug delivery systems, and tissue engineering scaffolds due to its biocompatibility and mechanical strength.
Recent work focuses on deciphering BcsP31’s role in the supramolecular organization of cellulose microfibrils and its potential as a genetic engineering target to enhance cellulose yield or modify material properties. Challenges include maintaining protein stability during recombinant expression and resolving its transient interactions within the dynamic Bcs complex. Ongoing studies employ techniques like cryo-EM, NMR, and mutagenesis to map functional domains. The development of BcsP31 variants through directed evolution or rational design represents an emerging frontier in synthetic biology approaches to tailor bacterial cellulose for specialized industrial applications.
×
