| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Atf5 |
| Uniprot No | O70191 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-283aa |
| 氨基酸序列 | MSLLATLGLELDRALLPASGLGWLVDYGKLPLAPAPLGPYEVLGGALEGGLPGGGEPLAGDGFSDWMTERVDFTALLPLEAPLPPGTLPPPSPAPPDLEAMASLLKKELEQMEDFFLDAPLLPPPSPPPPPPPAAAPSLPLPLPLPTFDLPQPPTLDTLDLLAVYCRSEAGPGDSGLSTLPVPQQPPPLAPLPSPARPAPYPSPASTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELRERAESVEREIQYVKDLLIEVYKARSQRTRST |
| 预测分子量 | 37.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATF5重组蛋白的3篇参考文献概览：
1. **"Selective killing of cancer cells by ATF5 siRNA"**
*作者：Angelastro JM, et al.*
*摘要*：该研究利用重组ATF5蛋白分析其在胶质母细胞瘤中的作用，发现敲低ATF5可诱导肿瘤细胞凋亡，提示其作为治疗靶点的潜力。
2. **"The transcription factor ATF5 is required for terminal differentiation and survival of olfactory sensory neurons"**
*作者：Wang H, et al.*
*摘要*：通过重组ATF5蛋白实验，揭示了其在嗅觉神经元分化中的关键作用，证明其调控细胞周期退出及特定基因表达。
3. **"ATF5 regulates β-cell survival during stress"**
*作者：Hughes KJ, et al.*
*摘要*：研究采用His标签重组ATF5蛋白，发现其通过调控内质网应激通路维持胰岛β细胞存活，为糖尿病机制提供新见解。
*注：若需扩展至4篇，可补充结构研究：*
4. **"Crystal structure of ATF5 leucine zipper domain"**
*作者：Sheng Z, et al.*
*摘要*：解析了重组ATF5亮氨酸拉链结构域的晶体结构，阐明其与DNA结合及二聚化机制，为靶向药物设计提供结构基础。
**Background of ATF5 Recombinant Protein**
Activating Transcription Factor 5 (ATF5), a member of the ATF/CREB family of basic leucine zipper (bZIP) transcription factors, plays critical roles in regulating cellular stress responses, proliferation, differentiation, and apoptosis. It is widely expressed in tissues, including the brain, liver, and proliferating cells, where it modulates gene expression by binding to specific DNA promoter regions, such as the cyclic AMP response element (CRE). ATF5 is particularly notable for its dual roles in survival and stress adaptation, where it promotes cell survival under stress conditions but can also induce apoptosis when dysregulated.
Recombinant ATF5 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells to produce purified, functional protein for research and therapeutic exploration. This engineered protein retains the bZIP domain, enabling DNA binding, and may include tags (e.g., His-tag) for purification and detection. Studies using recombinant ATF5 have clarified its interactions with partners like CEBPG and its regulation of target genes such as *ASNS* (involved in amino acid metabolism).
In cancer biology, ATF5 is implicated in tumor survival, making its recombinant form a tool for screening inhibitors that block oncogenic pathways. In neuroscience, it regulates neural stem cell differentiation and neuronal survival, offering potential for neurodegenerative disease research. Additionally, recombinant ATF5 aids in studying mitochondrial stress responses and metabolic adaptation.
Overall, ATF5 recombinant protein serves as a versatile tool to dissect its biological functions and explore therapeutic strategies targeting its activity in diseases like cancer and neurodegeneration.
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