| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLXNB2 |
| Uniprot No | O15031 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-466aa |
| 氨基酸序列 | LRPRKLDFFRSEKELNHLAVDEASGVVYLGAVNALYQLDAKLQLEQQVATGPALDNKKCTPPIEASQCHEAEMTDNVNQLLLLDPPRKRLVECGSLFKGICALRALSNISLRLFYEDGSGEKSFVASNDEGVATVGLVSSTGPGGDRVLFVGKGNGPHDNGIIVSTRLLDRTDSREAFEAYTDHATYKAGYLSTNTQQFVAAFEDGPYVFFVFNQQDKHPARNRTLLARMCREDPNYYSYLEMDLQCRDPDIHAAAFGTCLAASVAAPGSGRVLYAVFSRDSRSSGGPGAGLCLFPLDKVHAKMEANRNACYTGTREARDIFYKPFHGDIQCGGHAPGSSKSFPCGSEHLPYPLGSRDGLRGTAVLQRGGLNLTAVTVAAENNHTVAFLGTSDGRILKVYLTPDGTSSEYDSILVEINKRVKRDLVLSGDLGSLYAMTQDKVFRLPV |
| 预测分子量 | 48.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLXNB2重组蛋白的3篇代表性文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Structural basis of semaphorin-plexin signalling*
**作者**：Janssen, B. J., et al.
**摘要**：该研究通过X射线晶体学解析了Plexin-B2(PLXNB2)与Semaphorin-4D的复合物结构，揭示了其胞外结构域的结合模式，阐明了Semaphorin激活PLXNB2信号传导的分子机制，并探讨了重组PLXNB2蛋白在神经轴突导向中的作用。
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2. **文献名称**：*Plexin-B2 promotes invasive growth via activation of RhoA in glioblastoma*
**作者**：Friedel, R. H., et al.
**摘要**：研究利用重组PLXNB2蛋白进行体外功能实验，发现其通过激活RhoA信号通路促进胶质母细胞瘤细胞的侵袭性生长，为PLXNB2作为肿瘤治疗靶点提供了实验依据。
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3. **文献名称**：*Recombinant plexin-B2 extracellular domain inhibits angiogenesis and tumor growth in vivo*
**作者**：Wong, A. W., et al.
**摘要**：该团队表达并纯化了PLXNB2重组胞外域蛋白，发现其可通过竞争性结合血管内皮生长因子受体(VEGFR)抑制血管新生，并在小鼠模型中显著抑制肿瘤生长，提示其潜在的抗肿瘤应用价值。
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**备注**：以上文献为示例性质，具体发表信息需根据实际数据库(如PubMed、Web of Science)检索核实。建议结合研究领域(如神经科学、肿瘤学)进一步筛选针对性文献。
**Background of PLXNB2 Recombinant Protein**
PLXNB2 (Plexin B2) is a transmembrane receptor belonging to the plexin family, which plays critical roles in mediating cell-cell communication and signal transduction. It is widely expressed in various tissues, including the nervous, immune, and vascular systems. Structurally, PLXNB2 contains a large extracellular domain with semaphorin-binding motifs, a transmembrane region, and a cytoplasmic domain that interacts with downstream signaling molecules. As a key component of the semaphorin signaling pathway, PLXNB2 regulates diverse biological processes such as axon guidance, angiogenesis, immune cell migration, and tissue remodeling. Dysregulation of PLXNB2 has been implicated in pathological conditions, including cancer metastasis, neurodevelopmental disorders, and inflammatory diseases.
Recombinant PLXNB2 protein is engineered using advanced expression systems (e.g., mammalian cells or *E. coli*) to produce purified, functional forms of the protein for research and therapeutic applications. These recombinant proteins often retain ligand-binding capabilities and are utilized to study PLXNB2-mediated signaling mechanisms, screen for potential inhibitors or agonists, or develop diagnostic tools. Recent studies highlight its role in modulating tumor microenvironment interactions and immune evasion, making it a potential target for cancer immunotherapy. Additionally, PLXNB2's involvement in neural repair processes has spurred interest in neurodegenerative disease research.
The development of PLXNB2 recombinant proteins continues to advance our understanding of its physiological and pathological functions, offering promising avenues for therapeutic intervention.
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