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Recombinant Human SERPINE3 protein

  • 中文名: 丝氨酸蛋白酶抑制蛋白E3(SERPINE3)重组蛋白
  • 别    名: SERPINE3;Serpin E3
货号: PA2000-5200
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点SERPINE3
Uniprot No A8MV23
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间21-424aa
氨基酸序列HLREGMTLLKTEFALHLYQSVAACRNETNFVISPAGVSLPLEILQFGAEGSTGQQLADALGYTVHDKRVKDFLHAVYATLPTSSQGTEMELACSLFVQVGTPLSPCFVEHVSWWANSSLEPADLSEPNSTAIQTSEGASRETAGGGPSEGPGGWPWEQVSAAFAQLVLVSTMSFQGTWRKRFSSTDTQILPFTCAYGLVLQVPMMHQTTEVNYGQFQDTAGHQVGVLELPYLGSAVSLFLVLPRDKDTPLSHIEPHLTASTIHLWTTSLRRARMDVFLPRFRIQNQFNLKSILNSWGVTDLFDPLKANLKGISGQDGFYVSEAIHKAKIEVLEEGTKASGATALLLLKRSRIPIFKADRPFIYFLREPNTGITVFFDRIQIIYQCLSSNKGSFVHYPLKNKHSF
预测分子量51.6 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于SERPINE3重组蛋白的模拟参考文献示例(注:部分内容可能为模拟生成,非真实文献):

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1. **文献名称**: *SERPINE3 regulates trophoblast invasion and spiral artery remodeling in early pregnancy*

**作者**: Zhang L, et al.

**摘要**: 本研究揭示了SERPINE3在胎盘滋养层细胞侵袭和子宫螺旋动脉重塑中的关键作用。通过重组蛋白实验证实,SERPINE3通过抑制纤溶酶原激活剂(uPA)调控细胞外基质降解,影响滋养层细胞的迁移能力。

2. **文献名称**: *Recombinant SERPINE3 protein attenuates hepatic fibrosis via TGF-β/Smad signaling inhibition*

**作者**: Tanaka K, et al.

**摘要**: 研究利用大肠杆菌表达系统获得重组SERPINE3蛋白,并发现其能特异性结合TGF-β1.阻断Smad2/3磷酸化,显著减轻小鼠模型中的肝纤维化病理进程。

3. **文献名称**: *SERPINE3 promotes pancreatic cancer metastasis by modulating plasmin-mediated extracellular matrix remodeling*

**作者**: Chen H, et al.

**摘要**: 本文证明SERPINE3在胰腺癌中高表达,重组SERPINE3蛋白通过增强纤溶酶活性促进癌细胞侵袭。敲低SERPINE3可抑制小鼠模型中肿瘤的远处转移。

4. **文献名称**: *Expression and functional characterization of recombinant SERPINE3 in Drosophila models*

**作者**: Müller R, et al.

**摘要**: 该研究优化了SERPINE3在果蝇S2细胞中的分泌表达系统,发现其重组蛋白可调控果蝇胚胎发育过程中的丝氨酸蛋白酶网络,提示其进化保守功能。

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注:以上文献信息为基于领域知识的模拟生成,实际文献需通过PubMed/Google Scholar等平台以“SERPINE3”、“Serpin E3”、“重组蛋白”等关键词检索确认。

背景信息

SERPINE3 (Serpin Family E Member 3), also known as protease inhibitor 3 (PAI-3) or protein C inhibitor, is a member of the serpin superfamily of serine protease inhibitors. It plays a regulatory role in various physiological processes, including blood coagulation, fibrinolysis, cell migration, and inflammation. Structurally, SERPINE3 contains a conserved serpin domain that enables its interaction with target proteases, forming stable complexes to inhibit their enzymatic activity. It is expressed in multiple tissues, with notable presence in the liver, kidneys, and reproductive organs, and is regulated by hormonal signals, particularly androgens.

Recombinant SERPINE3 protein is engineered using expression systems such as bacteria, yeast, or mammalian cells to produce a purified, biologically active form. This recombinant version retains the functional properties of the native protein, allowing researchers to study its interactions with proteases like thrombin, activated protein C, and urokinase-type plasminogen activator (uPA). Its role in modulating protease activity has implications in pathological conditions, including thrombosis, cancer metastasis, and tissue fibrosis. For instance, SERPINE3 overexpression has been linked to tumor progression by promoting extracellular matrix remodeling and cell invasion.

In research, recombinant SERPINE3 is utilized to investigate mechanisms of hemostasis, inflammation, and tissue repair. It also serves as a tool for developing therapeutic strategies targeting protease-dependent pathways. Recent studies explore its dual role as both an inhibitor and a signaling molecule in cellular stress responses. Despite its potential, the protein’s pleiotropic effects and tissue-specific functions require further characterization, highlighting the importance of recombinant SERPINE3 in advancing biomedical research and drug discovery.

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