| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | sucC |
| Uniprot No | A0Q5H4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-387aa |
| 氨基酸序列 | MNLHEYQAKDLLESYGLKVQKGIVAHNPNEAAQAFDQLGGKFAVVKAQVHAGGRGKAGGVKVVKSSQEAREVAESLIGKNLVTFQTDAEGQPVNSVGVFEDVYPVTRELYLGAVVDRSSRKVTFMASTEGGVDIEEVAHNSPEKILKVEVDPLVGLQPFQAREVAFKLGLEGKQINDFVKTMLGAYKAFIECDFALFEINPLAVRENGEIVCVDGKINLDSNALYRHPKLLALRDKSQENAKELKASEHELNYVALEGNIGCMVNGAGLAMATMDIIQLYGGKPANFLDVGGGATKERVIEAFKLILDDENVKAVLINIFGGIVRCDMIAEAIIEAVKEVNVTVPVVVRLEGNNAEKGAKILADSGLKLIPADGLADAADKVVKSLG |
| 预测分子量 | 49.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于sucC重组蛋白研究的参考文献摘要概括(基于公开文献特征模拟，非真实文献)：
1. **《Cloning and expression of sucC gene in Escherichia coli for succinyl-CoA synthetase production》**
- **作者**: Zhang et al.
- **摘要**: 研究通过PCR扩增大肠杆菌sucC基因，构建重组质粒并在E. coli BL21中诱导表达，验证了重组琥珀酰辅酶A合成酶β亚基的可溶性表达及ATP依赖性酶活性。
2. **《Purification and functional characterization of recombinant SucC protein in Bacillus subtilis》**
- **作者**: Lee & Kim
- **摘要**: 利用枯草芽孢杆菌表达系统表达His标签融合的SucC蛋白，通过镍柱纯化获得高纯度蛋白，并证实其与SucD亚基协同催化琥珀酰-CoA生成的功能。
3. **《Structural insights into the catalytic mechanism of SucC in the TCA cycle》**
- **作者**: Müller et al.
- **摘要**: 通过X射线晶体学解析重组SucC蛋白的三维结构，揭示了其与底物结合的关键氨基酸残基及ATP/ADP结合位点，为靶向代谢酶的抑制剂设计提供依据。
4. **《Metabolic engineering of sucC-overexpressing strains for enhanced succinate production》**
- **作者**: Wang et al.
- **摘要**: 过表达sucC重组蛋白的工程菌株在三羧酸循环中显著提高琥珀酸合成效率，为微生物发酵生产琥珀酸的工业应用奠定基础。
(注：以上内容为模拟文献示例，实际文献需通过PubMed/Google Scholar等平台以"sucC recombinant protein"为关键词检索。)
**Background of SucC Recombinant Protein**
The *sucC* gene encodes the β-subunit of succinyl-CoA synthetase (SCS), a key enzyme in the tricarboxylic acid (TCA) cycle. SCS catalyzes the reversible conversion of succinyl-CoA to succinate, coupled with substrate-level phosphorylation to generate GTP or ATP, depending on the organism. This enzyme is critical for cellular energy production and intermediary metabolism. In prokaryotes like *Escherichia coli*, SCS consists of two subunits (α and β), encoded by *sucD* and *sucC*, respectively, forming a heterodimer essential for aerobic growth. Eukaryotic orthologs, localized in mitochondria, share structural and functional conservation.
Recombinant SucC protein is produced via heterologous expression systems (e.g., *E. coli*, yeast) for structural, biochemical, and functional studies. Its purification enables detailed analysis of enzymatic mechanisms, substrate specificity, and regulatory roles in metabolic pathways. Studies on SucC have also explored its potential in metabolic engineering, such as optimizing TCA flux for bioproduction. Additionally, mutations in SCS subunits are linked to metabolic disorders in humans, making recombinant SucC valuable for modeling mitochondrial diseases or drug screening.
Challenges in producing functional SucC include maintaining proper folding and post-translational modifications, often addressed through codon optimization, chaperone co-expression, or modified purification protocols. Structural studies (e.g., X-ray crystallography) of recombinant SucC have elucidated active-site architecture and conformational dynamics, aiding inhibitor design. Overall, SucC recombinant protein serves as a vital tool for dissecting energy metabolism and developing therapeutic or biotechnological applications.
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