| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | mcr-8 |
| Uniprot No | A0A2U7XX87 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 177-565aa |
| 氨基酸序列 | KDYASLIRNNMQIKDQALPFNFVRNTNGYLKRKYQASSTILQSVGEDAVRPIYSNAPPKLVVVVVGETARAQNFQLNGYSRVTNPYLSRRHDVISFKNVSSCGTATAISLPCMFSRMSRNEYNEVRAASEENLLDILKRTGVEVLWRNNNNGGCKGICKRVPTDDMPAMKVIGECVNKDGTCFDEVLLNQLSSRINAMQGDALIVLHQMGSHGPTYFERYPSTSKVFSPTCDSNLIEKCSNKELVNTYDNTLVYTDRMLSKTIELLQRYSGMRDVAMIYLSDHGESLGESGIYLHGTPYIIAPNEQTHIPMFMWFSSSFAQHSKLNLECLTGNADKQYSHDNFYHSILGLFNVKTSVYKPELDMFTLCRQSDHTPLSSAVVREKTDGNG |
| 预测分子量 | 46.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCR-8重组蛋白的参考文献示例(注：部分内容为示例性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**：*Functional Characterization of MCR-8. a Novel Phosphoethanolamine Transferase Conferring Plasmid-Mediated Colistin Resistance*
**作者**：Li R, Xie M, Zhang J, et al.
**摘要**：本研究首次报道了MCR-8重组蛋白的功能，通过在大肠杆菌中异源表达，证实其通过修饰细菌脂多糖的脂质A部分，赋予粘菌素抗性。酶活性实验表明，MCR-8与MCR-1具有相似的磷酸乙醇胺转移酶活性，但宿主适应性存在差异。
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2. **文献名称**：*Structural and Mechanistic Insights into MCR-8-Mediated Colistin Resistance*
**作者**：Wang C, Feng Y, Liu L, et al.
**摘要**：通过X射线晶体学解析MCR-8重组蛋白的三维结构，揭示其催化口袋的关键残基。结合分子动力学模拟，提出MCR-8与底物结合的分子机制，为抑制剂的开发提供结构基础。
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3. **文献名称**：*Heterologous Expression and Biochemical Analysis of MCR-8 in Multidrug-Resistant Enterobacteriaceae*
**作者**：Yang Y, Qiu Y, Hsueh PR, et al.
**摘要**：在多重耐药肠杆菌科中表达重组MCR-8蛋白，发现其可与其他耐药基因(如NDM-1)协同作用，显著提高细菌对粘菌素的最小抑菌浓度(MIC)。研究强调了MCR-8在临床耐药性传播中的潜在风险。
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4. **文献名称**：*Comparative Genomics of mcr-8-Harboring Plasmids and Role in Colistin Resistance Dissemination*
**作者**：Bonnin RA, Poirel L, Nordmann P.
**摘要**：分析了携带mcr-8的质粒遗传背景，发现其常与移动遗传元件(如IS)相关联。通过重组蛋白表达实验，验证了MCR-8在不同宿主中的功能性，提示质粒介导的横向基因转移是耐药性扩散的主要途径。
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**备注**：以上文献为示例性质，实际研究中请通过PubMed、Web of Science等平台以“MCR-8”、“phosphoethanolamine transferase”、“colistin resistance”等关键词检索最新文献。
The MCR-8 recombinant protein is derived from the mobile colistin resistance gene *mcr-8*, a member of the MCR phosphoethanolamine transferase family. First identified in *Klebsiella pneumoniae* and *Escherichia coli*, MCR enzymes mediate resistance to colistin, a last-resort antibiotic for multidrug-resistant Gram-negative infections. MCR-8. like other MCR variants (e.g., MCR-1 to MCR-10), catalyzes the addition of phosphoethanolamine to lipid A in bacterial lipopolysaccharides, reducing colistin’s binding affinity and conferring resistance. Its emergence highlights the global spread of plasmid-borne polymyxin resistance, threatening public health.
Recombinant MCR-8 proteins are engineered for functional and structural studies. By cloning and expressing the *mcr-8* gene in heterologous systems (e.g., *E. coli*), researchers purify the protein to analyze its enzymatic activity, inhibition mechanisms, and interactions with colistin. Structural insights from X-ray crystallography or cryo-EM help design inhibitors to counteract resistance. Studies also explore MCR-8’s genetic context, transmission dynamics, and co-occurrence with other resistance genes, informing surveillance strategies.
The rise of MCR-8 underscores the urgent need for novel antimicrobials and stewardship programs. As colistin remains critical in treating carbapenem-resistant infections, understanding MCR enzymes through recombinant models is vital for combating antibiotic resistance, a priority emphasized by the WHO. Research on MCR-8 contributes to global efforts in tracking resistance evolution and developing targeted therapies.
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