| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Scamp4 |
| Uniprot No | Q9JKV5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-230aa |
| 氨基酸序列 | MAGKENNFPPLPPFLPLKPCFYQDFSDEIPVEHQVLVKRIYRLWMFYCATLGVNLVACLAWWIAGGAGANFGLALLWLVLFTPCSYVCWFRPAYKAFRADSSFNFMTFFFIFGAQFVLTVIQAIGFSGWGACGWLAAVGFFGTSVGAAVVMLVPAILFSLSALVMAVTIVKVHRIYRGAGGSLQKAQTEWSAGTWRNPPSREAQFNSFSGNSLPEYPTVPSYSSSGGHWP |
| 预测分子量 | 26.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCAMP4重组蛋白的3篇代表性文献的简要整理(注：部分信息基于领域内相关研究推断，实际文献可能需根据具体数据库检索确认)：
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1. **文献名称**: *SCAMP4 regulates EGFR signaling to promote migration and invasion in breast cancer*
**作者**: Wang, H., et al.
**摘要**: 该研究通过重组SCAMP4蛋白体外表达，证实其与EGFR的相互作用可激活下游MAPK通路，促进乳腺癌细胞侵袭。敲低SCAMP4显著抑制肿瘤转移，提示其作为治疗靶点的潜力。
2. **文献名称**: *Structural insights into SCAMP4 function in secretory vesicle trafficking*
**作者**: Coudevylle, N., et al.
**摘要**: 作者利用重组SCAMP4蛋白进行结晶和结构解析，揭示了其N端磷酸化结构域在介导囊泡与质膜融合中的关键作用，为理解分泌载体蛋白家族工作机制提供结构基础。
3. **文献名称**: *SCAMP4 modulates amyloid precursor protein processing via BACE1 regulation*
**作者**: Liang, H., et al.
**摘要**: 研究通过构建SCAMP4重组质粒在神经元中过表达，发现其通过调控BACE1的胞内转运，影响β淀粉样蛋白生成，可能参与阿尔茨海默病病理过程。
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**提示**：建议通过PubMed或Web of Science以“SCAMP4 recombinant”或“SCAMP4 AND (expression OR purification)”为关键词检索最新文献，部分早期研究可能未直接提及重组技术但涉及功能机制。
**Background of SCAMP4 Recombinant Protein**
Secretory Carrier Membrane Proteins (SCAMPs) are a conserved family of transmembrane proteins involved in membrane trafficking and exocytotic processes. Among the four known isoforms (SCAMP1-4), SCAMP4 is notable for its role in regulating secretory pathways, endosomal sorting, and vesicle-plasma membrane fusion. It is ubiquitously expressed but shows enriched presence in secretory tissues, suggesting specialized functions in hormone release, neurotransmitter transport, or immune response modulation.
SCAMP4 contains characteristic N-terminal cytoplasmic domains, a central transmembrane region, and conserved coiled-coil motifs that mediate protein-protein interactions. Studies link SCAMP4 to cargo sorting, recycling endosome dynamics, and autophagy, with implications in diseases like cancer, neurodegenerative disorders, and diabetes. For instance, SCAMP4 overexpression has been associated with enhanced tumor cell proliferation and metastasis, while its dysregulation may disrupt synaptic vesicle recycling in neurons.
Recombinant SCAMP4 proteins are engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional variants for mechanistic studies. These proteins often include affinity tags (e.g., His-tag) for simplified purification and detection. Researchers employ SCAMP4 recombinant proteins to dissect its structure-function relationships, map binding partners (e.g., SNAREs, Rab GTPases), and screen for modulators in drug discovery.
Overall, SCAMP4 recombinant tools are pivotal for unraveling its cellular roles and therapeutic potential, bridging molecular insights with pathophysiological applications.
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