| 纯度 | >85% (SDS-PAGE) |
| 种属 | Human |
| 靶点 | SERPINF2 |
| Uniprot No | P08697 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | MVLLWGLLVLSWSCLQGPCSVFSPVSAMEPLGRQLTSGPNQEQVSPLTLL KLGNQEPGGQTALKSPPGVCSRDPTPEQTHRLARAMMAFTADLFSLVAQT STCPNLILSPLSVALALSHLALGAQNHTLQRLQQVLHAGSGPCLPHLLSR LCQDLGPGAFRLAARMYLQKGFPIKEDFLEQSEQLFGAKPVSLTGKQEDD LANINQWVKEATEGKIQEFLSGLPEDTVLLLLNAIHFQGFWRNKFDPSLT QRDSFHLDEQFTVPVEMMQARTYPLRWFLLEQPEIQVAHFPFKNNMSFVV LVPTHFEWNVSQVLANLSWDTLHPPLVWERPTKVRLPKLYLKHQMDLVAT LSQLGLQELFQAPDLRGISEQSLVVSGVQHQSTLELSEVGVEAAAATSIA MSRMSLSSFSVNRPFLFFIFEDTTGLPLFVGSVRNPNPSAPRELKEQQDS PGNKDFLQSLKGFPRGDKLFGPDLKLVPPMEEDYPQFGSPK |
| 预测分子量 | 81 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SERPINF2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Recombinant expression and functional characterization of human SERPINF2 (alpha-2 antiplasmin) in HEK293 cells*"
**作者**：Chen L, Wang X, et al.
**摘要**：研究报道了在HEK293细胞中成功表达具有生物活性的重组SERPINF2蛋白，并通过体外实验验证其抑制纤溶酶的能力，为治疗遗传性纤溶亢进疾病提供了潜在候选药物。
2. **文献名称**：*"Structural and functional analysis of a novel SERPINF2 variant linked to bleeding disorders*"
**作者**：Garcia-Ruiz C, Morales V, et al.
**摘要**：通过重组蛋白技术获得突变型SERPINF2.发现其与纤溶酶的结合能力显著下降，揭示了该蛋白特定结构域在调控凝血功能中的关键作用。
3. **文献名称**：*"High-yield production of bioactive SERPINF2 in Pichia pastoris: Implications for therapeutic applications*"
**作者**：Kim S, Park JH, et al.
**摘要**：利用毕赤酵母表达系统高效生产重组SERPINF2.优化后的工艺显著提高了蛋白产量和纯度，并证明其在动物模型中有效减少血栓溶解后的过度出血。
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注：上述文献为示例，实际引用时需根据真实发表的论文调整信息。建议通过PubMed或Web of Science以关键词“SERPINF2 recombinant”或“alpha-2 antiplasmin expression”检索最新研究。
SERPINF2. also known as alpha-2-antiplasmin (α2AP), is a serine protease inhibitor encoded by the SERPINF2 gene. It plays a critical role in regulating fibrinolysis, the process that prevents excessive blood clot formation by breaking down fibrin networks. As the primary physiological inhibitor of plasmin, SERPINF2 binds irreversibly to plasmin, neutralizing its enzymatic activity and maintaining a balance between clot formation and dissolution. This glycoprotein is synthesized primarily in the liver and circulates in plasma, with a portion cross-linked to fibrin clots during coagulation to provide localized inhibition of fibrinolysis.
Recombinant SERPINF2 protein is produced using biotechnological platforms such as mammalian cell expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications, particularly glycosylation, which is essential for its stability and functional activity. Researchers utilize recombinant SERPINF2 to study fibrinolysis dysregulation in conditions like bleeding disorders (e.g., congenital α2AP deficiency), thrombotic diseases, or trauma-associated coagulopathy. Its therapeutic potential is being explored for managing hemorrhagic complications or enhancing clot stability in hemophilia patients.
Recent studies also investigate SERPINF2's non-hemostatic roles, including its involvement in tissue remodeling, angiogenesis, and tumor progression. Structural analyses of recombinant SERPINF2 help elucidate its reactive center loop mechanism and interactions with plasmin. However, challenges remain in optimizing production yields and maintaining consistent glycosylation patterns for clinical applications. As a regulator at the intersection of coagulation, inflammation, and cellular signaling, recombinant SERPINF2 continues to be a valuable tool for both basic research and therapeutic development.
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