| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMEM158 |
| Uniprot No | Q8WZ71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-300aa |
| 氨基酸序列 | GAADAPGLLGVPSNASVNASSADEPIAPRLLASAAPGPPERPGPEEAAAAAAPCNISVQRQMLSSLLVRWGRPRGFQCDLLLFSTNAHGRAFFAAAFHRVGPPLLIEHLGLAAGGAQQDLRLCVGCGWVRGRRTGRLRPAAAPSAAAATAGAPTALPAYPAAEPPGPLWLQGEPLHFCCLDFSLEELQGEPGWRLNRKPIESTLVACFMTLVIVVWSVAALIWPVPIIAGFLPNGMEQRRTTASTTAATPAAVPAGTTAAAAAAAAAAAAAAVTSGVATK |
| 预测分子量 | 29.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TMEM158重组蛋白的假设性参考文献示例(仅供参考，建议通过学术数据库核实真实文献)：
1. **文献名称**: "TMEM158 promotes tumor progression in glioblastoma via TGF-β signaling"
**作者**: Smith A, et al.
**摘要**: 研究揭示TMEM158在胶质母细胞瘤中高表达，通过激活TGF-β通路促进肿瘤细胞侵袭和化疗耐药，重组蛋白实验证实其调控EMT过程。
2. **文献名称**: "TMEM158 as a stress-induced regulator of dopaminergic neuron survival in Parkinson's disease"
**作者**: Zhang Y, et al.
**摘要**: 发现TMEM158在帕金森病模型中受氧化应激诱导表达，重组蛋白过表达加剧多巴胺能神经元凋亡，提示其可能通过线粒体途径参与神经退行机制。
3. **文献名称**: "High-yield purification and characterization of recombinant TMEM158 for structural studies"
**作者**: Lee HJ, et al.
**摘要**: 报道一种新型大肠杆菌表达系统，通过His标签纯化获得高稳定性TMEM158重组蛋白，并利用质谱和圆二色谱解析其二级结构特征。
4. **文献名称**: "TMEM158: A novel biomarker in triple-negative breast cancer linked to poor prognosis"
**作者**: Johnson R, et al.
**摘要**: 基于重组TMEM158的体外实验显示，其通过RAS/MAPK通路增强乳腺癌细胞增殖，临床数据分析证实其高表达与患者生存期缩短显著相关。
**注意**：以上为模拟示例，实际文献需在PubMed、Web of Science等平台以"TMEM158 recombinant protein"或"TMEM158 overexpression"为关键词检索。真实研究可能集中于其在癌症、衰老或再生医学中的功能机制。
**Background of TMEM158 Recombinant Protein**
TMEM158 (Transmembrane Protein 158), also known as RIS1 or STARD3 co-regulated gene, is a protein encoded by the *TMEM158* gene, located on human chromosome 3p21.1. It is a conserved transmembrane protein implicated in diverse cellular processes, including cell proliferation, apoptosis, and stress responses. TMEM158 has garnered attention for its dual roles in cancer and neurodegenerative diseases. In cancer, it is frequently upregulated in glioblastoma, pancreatic cancer, and other malignancies, where it promotes tumor growth by modulating MAPK/ERK signaling or interacting with oncogenic pathways. Conversely, in Parkinson’s disease, reduced TMEM158 expression correlates with neuronal degeneration, suggesting a protective role in dopaminergic neurons.
The recombinant TMEM158 protein is engineered using expression systems like *E. coli* or mammalian cells to produce soluble, bioactive forms for functional studies. Its structure includes an N-terminal signal peptide, a transmembrane domain, and a C-terminal region with potential glycosylation sites, which may influence its stability and interactions. Recombinant variants often incorporate tags (e.g., His-tag) for purification and detection.
Research applications of TMEM158 recombinant protein span *in vitro* assays to elucidate its molecular mechanisms, antibody development for diagnostic biomarkers, and drug screening for therapies targeting TMEM158-associated diseases. Its role in stress granules and autophagy further links it to cellular adaptation pathways. Despite progress, questions remain about its precise molecular partners and context-dependent functions, driving ongoing studies to harness its therapeutic potential.
×
