| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Syt12 |
| Uniprot No | Q8IV01 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-421aa |
| 氨基酸序列 | MAVDVAEYHLSVIKSPPGWEVGVYAAGALALLGIAAVSLWKLWTSGSFPSPSPFPNYDYRYLQQKYGESCAEAREKRVPAWNAQRASTRGPPSRKGSLSIEDTFESISELGPLELMGRELDLAPYGTLRKSQSADSLNSISSVSNTFGQDFTLGQVEVSMEYDTASHTLNVAVMQGKDLLEREEASFESCFMRVSLLPDEQIVGISRIQRNAYSIFFDEKFSIPLDPTALEEKSLRFSVFGIDEDERNVSTGVVELKLSVLDLPLQPFSGWLYLQDQNKAADAVGEILLSLSYLPTAERLTVVVVKAKNLIWTNDKTTADPFVKVYLLQDGRKMSKKKTAVKRDDPNPVFNEAMIFSVPAIVLQDLSLRVTVAESSSDGRGDNVGHVIIGPSASGMGTTHWNQMLATLRRPVSMWHAVRRN |
| 预测分子量 | 46.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Syt12(Synaptotagmin-12)重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Synaptotagmin XII interacts with the voltage-sensitive calcium channel in photoreceptor terminals"*
**作者**: Wang J, et al.
**摘要**: 该研究利用重组Syt12蛋白探究其与光感受器细胞中电压敏感钙通道的相互作用，发现Syt12可能通过调控钙离子内流参与突触传递的调节。
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2. **文献名称**: *"Expression and functional characterization of recombinant Synaptotagmin-12 in neuroendocrine cells"*
**作者**: Lee SJ, Kim H.
**摘要**: 作者通过哺乳动物表达系统制备重组Syt12蛋白，发现其在神经内分泌细胞中促进囊泡分泌，并证明其钙离子结合结构域对功能至关重要。
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3. **文献名称**: *"Structural insights into the calcium-independent membrane binding properties of Synaptotagmin-12"*
**作者**: Chen X, et al.
**摘要**: 通过重组Syt12蛋白的结构分析，揭示了其C2结构域在无钙条件下的膜结合能力，表明Syt12可能参与非典型突触传递或细胞膜修复过程。
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注：以上文献为虚拟示例，实际研究中建议通过PubMed或Google Scholar检索具体论文。Syt12研究相对较少，可扩展关键词(如“synaptotagmin家族”“重组蛋白表达”)获取更多资料。
**Background of Syt12 Recombinant Protein**
Synaptotagmin-12 (Syt12), a member of the synaptotagmin family, is a calcium-binding protein implicated in regulating membrane trafficking and synaptic transmission. This family comprises 17 isoforms in mammals, with Syt12 distinguished by its expression in non-neuronal tissues, such as the pancreas and kidney, alongside the central nervous system. Structurally, Syt12 features two conserved C2 domains (C2A and C2B) that mediate calcium-dependent interactions with phospholipids and SNARE proteins, though its calcium sensitivity differs from canonical isoforms like Syt1.
Functionally, Syt12 is linked to modulating exocytosis and intracellular signaling. Studies suggest its role in insulin secretion in pancreatic β-cells and dopamine release in neurons, highlighting tissue-specific regulatory mechanisms. Its recombinant form is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable biochemical and functional studies. Recombinant Syt12 allows researchers to explore its calcium-dependent binding properties, structural dynamics, and interactions with partner proteins in vitro.
Interest in Syt12 also stems from its potential involvement in diseases. Dysregulation has been observed in neurological disorders, such as Parkinson’s disease, and certain cancers, where aberrant exocytosis or signaling may contribute to pathogenesis. Additionally, Syt12’s unique tissue distribution positions it as a candidate biomarker or therapeutic target. Current research focuses on clarifying its physiological roles, elucidating its regulatory networks, and exploiting recombinant Syt12 for drug screening or diagnostic applications. Its study bridges gaps in understanding calcium-dependent secretion mechanisms across diverse cell types.
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