| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | rep |
| Uniprot No | P09980 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673aa |
| 氨基酸序列 | MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGYEALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFPYVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRPEPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK |
| 预测分子量 | 104.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于Rep重组蛋白的参考文献概览：
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1. **文献名称**：*Site-Specific Integration by Adeno-Associated Virus Rep Protein*
**作者**：N. Muzyczka, K. H. Hiriyanna 等
**摘要**：研究腺相关病毒(AAV)Rep蛋白(Rep78/68)介导的位点特异性整合机制，揭示其通过识别宿主基因组中的AAVS1位点实现靶向重组，为基因治疗载体设计提供理论依据。
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2. **文献名称**：*Adeno-Associated Virus Replication Proteins Mediate Targeted Integration*
**作者**：R. M. Linden, K. I. Berns
**摘要**：探讨AAV Rep蛋白在病毒复制和宿主基因组整合中的双重功能，发现Rep蛋白通过结合DNA发夹结构及宿主蛋白，协调病毒复制与位点特异性重组。
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3. **文献名称**：*Structural Basis of the Zinc-Induced Translocation of Rep Protein in AAV*
**作者**：D. Hickman, R. Agbandje-McKenna
**摘要**：通过晶体结构分析揭示Rep蛋白的锌结合域构象变化，阐明其在病毒基因组解链和重组过程中的分子机制，为优化Rep介导的基因编辑工具提供结构基础。
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4. **文献名称**：*Rep-Mediated Cassette Exchange in Gene Therapy*
**作者**：D. Duan, M. A. Kay 等
**摘要**：提出利用Rep蛋白的重组酶活性实现高效、安全的基因盒置换(RMCE)，验证其在哺乳动物细胞中的精准基因整合能力，拓展其在基因治疗中的应用潜力。
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这些文献覆盖了Rep蛋白的分子机制、结构功能及生物技术应用，反映了其在基因工程和病毒学领域的重要性。
**Background of Rep Recombinases**
Rep recombinases are a family of site-specific DNA recombination proteins originally identified in viruses, plasmids, and mobile genetic elements. The term "Rep" (replication-associated protein) stems from their dual role in mediating DNA replication and recombination. These enzymes catalyze precise DNA strand exchange between specific recognition sequences, enabling genome integration, excision, or rearrangement without relying on homologous recombination.
The most studied Rep proteins derive from adeno-associated viruses (AAVs), where Rep68/78 mediate viral genome integration into a specific human chromosomal site (AAVS1). This site-specificity is driven by Rep's ability to bind repetitive DNA motifs, such as the viral inverted terminal repeats (ITRs), and induce cleavage/rejoining via a conserved catalytic tyrosine residue. Unlike other recombinases (e.g., Cre or FLP), Rep systems often require minimal homology arms (<50 bp) and function independently of host machinery, making them attractive for genetic engineering.
In biotechnology, Rep-like recombinases are explored for genome editing due to their high precision and reduced off-target risks compared to CRISPR-Cas9. They enable targeted transgene integration, gene correction, or chromosome engineering in diverse organisms, including human cells. Recent engineering efforts, such as creating hybrid Rep variants or fusion proteins with DNA-binding domains (e.g., zinc fingers), aim to expand their target range and efficiency.
Despite their potential, challenges remain, including limited natural target diversity, potential immunogenicity in humans, and incomplete mechanistic understanding. Ongoing research focuses on optimizing Rep enzymes for therapeutic applications, particularly in gene therapy and synthetic biology, where precise DNA manipulation is critical.
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