| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIOK3 |
| Uniprot No | O14730 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-519aa |
| 氨基酸序列 | MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE |
| 预测分子量 | 62.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RIOK3重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: *RIOK3 Phosphorylates IRF3 to Regulate Antiviral Innate Immunity*
**作者**: Smith A, et al.
**摘要**: 研究利用重组RIOK3蛋白进行体外激酶实验，发现其通过磷酸化干扰素调节因子3(IRF3)增强I型干扰素的产生，揭示了RIOK3在宿主抗病毒免疫应答中的关键作用。
2. **文献名称**: *RIOK3 Promotes Tumor Metastasis via EMT Pathway in Colorectal Cancer*
**作者**: Zhang L, et al.
**摘要**: 通过表达重组RIOK3蛋白并处理结肠癌细胞，研究发现其激活上皮-间质转化(EMT)信号通路，促进癌细胞迁移和侵袭，提示其作为癌症治疗潜在靶点。
3. **文献名称**: *Structural Insights into RIOK3 Kinase Domain and Its Role in Autophagy*
**作者**: Brown K, et al.
**摘要**: 研究解析了重组RIOK3激酶结构域的晶体结构，结合功能实验表明其通过调控自噬相关蛋白ATG5影响细胞自噬过程。
4. **文献名称**: *RIOK3 Interacts with SARS-CoV-2 N Protein to Modulate Viral Replication*
**作者**: Lee J, et al.
**摘要**: 利用重组RIOK3蛋白与病毒蛋白互作实验，发现其与SARS-CoV-2核衣壳蛋白(N)结合，可能通过干扰宿主RNA加工机制抑制病毒复制。
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**注**：以上内容为示例性模拟，实际文献需通过学术数据库(如PubMed、Web of Science)检索关键词“RIOK3 recombinant protein”或“RIOK3 kinase function”获取。如需真实文献，建议结合具体研究方向进一步筛选。
RIOK3 (RIO kinase 3) is a member of the RIO kinase family, a group of atypical serine/threonine kinases characterized by their conserved RIO (right open reading frame) domain. Unlike conventional kinases, RIO kinases lack regulatory domains and often exhibit unique catalytic mechanisms. RIOK3 is evolutionarily conserved and plays diverse roles in cellular processes, including ribosome biogenesis, cell cycle regulation, innate immune signaling, and cytoskeletal dynamics. Structurally, it contains a C-terminal kinase domain and an N-terminal region involved in protein-protein interactions. Dysregulation of RIOK3 has been implicated in cancer progression, inflammatory diseases, and viral infections, making it a potential therapeutic target.
Recombinant RIOK3 protein is engineered for research applications, typically produced using heterologous expression systems like *E. coli* or mammalian cell cultures. The recombinant form retains the kinase activity and structural features of native RIOK3. enabling *in vitro* studies on its enzymatic behavior, substrate specificity, and interaction partners. Researchers utilize it to investigate signaling pathways, particularly its role in activating NF-κB and TGF-β pathways, which are critical in inflammation and epithelial-mesenchymal transition (EMT) in cancer. Additionally, recombinant RIOK3 facilitates drug discovery efforts, such as screening kinase inhibitors or validating CRISPR/Cas9-mediated gene editing outcomes.
Studies highlight RIOK3's oncogenic potential, as its overexpression correlates with poor prognosis in cancers like breast, lung, and glioblastoma. It promotes tumor metastasis by enhancing cell migration, invasion, and resistance to apoptosis. In immune regulation, RIOK3 interacts with pattern recognition receptors (e.g., RIG-I) to modulate antiviral responses. Despite progress, questions remain about its precise molecular mechanisms and context-dependent functions, driving continued interest in recombinant protein-based studies. Quality-controlled batches of recombinant RIOK3 are essential for reproducibility in biochemical assays, structural studies (e.g., crystallography), and functional genomics.
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