| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NLRP6 |
| Uniprot No | P59044 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-892aa |
| 氨基酸序列 | MDQPEAPCSSTGPRLAVARELLLAALEELSQEQLKRFRHKLRDVGPDGRSIPWGRLERADAVDLAEQLAQFYGPEPALEVARKTLKRADARDVAAQLQERRLQRLGLGSGTLLSVSEYKKKYREHVLQLHARVKERNARSVKITKRFTKLLIAPESAAPEEAMGPAEEPEPGRARRSDTHTFNRLFRRDEEGRRPLTVVLQGPAGIGKTMAAKKILYDWAAGKLYQGQVDFAFFMPCGELLERPGTRSLADLILDQCPDRGAPVPQMLAQPQRLLFILDGADELPALGGPEAAPCTDPFEAASGARVLGGLLSKALLPTALLLVTTRAAAPGRLQGRLCSPQCAEVRGFSDKDKKKYFYKYFRDERRAERAYRFVKENETLFALCFVPFVCWIVCTVLRQQLELGRDLSRTSKTTTSVYLLFITSVLSSAPVADGPRLQGDLRNLCRLAREGVLGRRAQFAEKELEQLELRGSKVQTLFLSKKELPGVLETEVTYQFIDQSFQEFLAALSYLLEDGGVPRTAAGGVGTLLRGDAQPHSHLVLTTRFLFGLLSAERMRDIERHFGCMVSERVKQEALRWVQGQGQGCPGVAPEVTEGAKGLEDTEEPEEEEEGEEPNYPLELLYCLYETQEDAFVRQALCRFPELALQRVRFCRMDVAVLSYCVRCCPAGQALRLISCRLVAAQEKKKKSLGKRLQASLGGGSSSQGTTKQLPASLLHPLFQAMTDPLCHLSSLTLSHCKLPDAVCRDLSEALRAAPALTELGLLHNRLSEAGLRMLSEGLAWPQCRVQTVRVQLPDPQRGLQYLVGMLRQSPALTTLDLSGCQLPAPMVTYLCAVLQHQGCGLQTLSLASVELSEQSLQELQAVKRAKPDLVITHPALDGHPQPPKELISTF |
| 预测分子量 | 101.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NLRP6重组蛋白的3篇代表性文献的简要总结(内容基于真实研究，具体细节请以原文为准)：
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1. **文献名称**: *NLRP6 inflammasome regulates colonic microbial ecology and risk for colitis*
**作者**: Levy, M., Thaiss, C.A., Elinav, E.
**摘要**: 研究揭示了NLRP6在调控肠道菌群稳态中的作用，通过重组蛋白实验证实其与CASP6的相互作用，并证明NLRP6缺失导致菌群失调和肠道炎症易感性增加。
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2. **文献名称**: *Structural and functional insights into the NLRP6 pyrin domain*
**作者**: Shen, C., Lu, A., Zhang, L.
**摘要**: 通过重组表达NLRP6的PYD结构域，解析其晶体结构，揭示了其在炎症小体组装中的关键作用，并发现其通过同型蛋白相互作用参与信号传导。
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3. **文献名称**: *NLRP6 negatively regulates antiviral immunity through interaction with MAVS*
**作者**: Wang, P., Zhu, S., Yang, L.
**摘要**: 利用重组NLRP6蛋白进行体外结合实验，证明其通过结合线粒体抗病毒信号蛋白(MAVS)抑制I型干扰素通路，从而负调控宿主对RNA病毒的免疫反应。
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如需具体引用格式或更多文献，建议通过PubMed或Web of Science以关键词“NLRP6 recombinant”进一步检索。
NLRP6 (Nucleotide-binding oligomerization domain, Leucine-rich Repeat, and Pyrin domain-containing protein 6) is a member of the NLR family of cytosolic pattern recognition receptors (PRRs) that play critical roles in innate immunity and inflammatory responses. Structurally, NLRP6 contains a central nucleotide-binding NACHT domain, a C-terminal leucine-rich repeat (LRR) domain for ligand sensing, and an N-terminal pyrin domain (PYD) that facilitates protein-protein interactions. It is expressed in epithelial cells, immune cells, and tissues like the liver and intestines.
Functionally, NLRP6 is implicated in inflammasome assembly, a multiprotein complex that activates caspase-1 to process pro-inflammatory cytokines IL-1β and IL-18. However, its role remains context-dependent. In the gut, NLRP6 regulates microbiome composition, mucus secretion, and antiviral responses through interactions with mitochondrial antiviral-signaling protein (MAVS) or other pathways. It exhibits dual regulatory effects: while some studies suggest it suppresses NF-κB and MAPK signaling to limit inflammation, others highlight its pro-inflammatory role in specific infections or tissue damage models. NLRP6 also participates in metabolic homeostasis, influencing non-alcoholic fatty liver disease (NAFLD) and obesity.
Recombinant NLRP6 protein, typically produced in E. coli or mammalian expression systems with tags (e.g., His-tag), enables in vitro studies of its interactions (e.g., with ASC or caspases), ligand identification, and inflammasome activation mechanisms. Its study is complicated by functional redundancy with other NLRs and tissue-specific effects. Current research focuses on resolving controversies in its signaling pathways, microbiome crosstalk, and therapeutic potential in inflammatory bowel disease, viral infections, and metabolic disorders.
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