| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDCD11 |
| Uniprot No | Q14690 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1200-1420aa |
| 氨基酸序列 | GQALRATVVGPDSSKTLLCLSLTGPHKLEEGEVAMGRVVKVTPNEGLTVSFPFGKIGTVSIFHMSDSYSETPLEDFVPQKVVRCYILSTADNVLTLSLRSSRTNPETKSKVEDPEINSIQDIKEGQLLRGYVGSIQPHGVFFRLGPSVVGLARYSHVSQHSPSKKALYNKHLPEGKLLTARVLRLNHQKNLVELSFLPGDTGKPDVLSASLEGQLTKQEER |
| 预测分子量 | 25.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDCD11重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Recombinant PDCD11 Protein Induces Apoptosis in Cancer Cells via Caspase Activation"*
**作者**：Chen L, Wang X, et al.
**摘要**：本研究通过在大肠杆菌系统中表达PDCD11重组蛋白，发现其能显著激活Caspase-3/9通路，诱导肿瘤细胞凋亡，提示其在癌症治疗中的潜在应用。
2. **文献名称**：*"Structural and Functional Characterization of Human PDCD11 Recombinant Protein in Ribosome Assembly"*
**作者**：Miyazaki T, Ito T, et al.
**摘要**：利用杆状病毒-昆虫细胞系统表达PDCD11重组蛋白，结合冷冻电镜技术解析其三维结构，证实其通过WD40结构域参与核糖体RNA加工及组装过程。
3. **文献名称**：*"PDCD11 Recombinant Protein Enhances p53-Mediated Transcriptional Activity in DNA Damage Response"*
**作者**：Gupta S, Kumar P, et al.
**摘要**：通过哺乳动物细胞表达纯化PDCD11重组蛋白，发现其与p53蛋白直接互作，增强p53靶基因的转录活性，揭示了其在DNA损伤修复中的分子机制。
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**备注**：若需真实文献，建议在PubMed或Google Scholar中检索关键词“PDCD11 recombinant protein”或结合其别名(如RRP5)进行查询，并筛选涉及重组表达、功能分析的研究。
PDCD11 (Programmed Cell Death 11), also known as SPF1 or CRFG, is a conserved eukaryotic protein implicated in ribosome biogenesis, cell cycle regulation, and apoptosis. Encoded by the PDCD11 gene in humans, it localizes to the nucleolus and contains structural motifs such as a sigma70-like domain, suggesting roles in RNA binding or processing. Studies indicate PDCD11 participates in pre-rRNA maturation, interacting with components of the small subunit (SSU) processome to facilitate 18S rRNA synthesis. Its dysfunction disrupts ribosome assembly, leading to nucleolar stress and p53-mediated cell cycle arrest or apoptosis, linking it to tumor suppression pathways.
Recombinant PDCD11 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional forms for biochemical studies. This enables exploration of its molecular interactions, enzymatic activities, and structural properties. Research applications include analyzing its role in ribosomopathies, cancer (e.g., altered expression in hepatocellular carcinoma), and neurodegenerative diseases. Additionally, recombinant PDCD11 serves as an antigen for antibody development or a tool for screening therapeutic agents targeting ribosome biosynthesis pathways. Despite progress, mechanistic details of PDCD11's multifunctional roles remain under investigation, highlighting its relevance in both basic and translational research.
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