| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPD2 |
| Uniprot No | P43304 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 43-727aa |
| 氨基酸序列 | ADCISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSGCALDAVTRGLKTALVERDDFSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIMLPVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQHNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVRVSGARCKDVLTGQEFDVRAKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIFFLPWQKMTIAGTTDTPTDVTHHPIPSEEDINFILNEVRNYLSCDVEVRRGDVLAAWSGIRPLVTDPKSADTQSISRNHVVDISESGLITIAGGKWTTYRSMAEDTINAAVKTHNLKAGPSRTVGLFLQGGKDWSPTLYIRLVQDYGLESEVAQHLAATYGDKAFEVAKMASVTGKRWPIVGVRLVSEFPYIEAEVKYGIKEYACTAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELNWDDYKKQEQLETARKFLYYEMGYKSRSEQLTDRSEISLLPSDIDRYKKRFHKFDADQKGFITIVDVQRVLESINVQMDENTLHEILNEVDLNKNGQVELNEFLQLMSAIQKGRVSGSRLAILMKTAEENLDRRVPIPVDRSCGGL |
| 预测分子量 | 81.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPD2重组蛋白的3篇参考文献概述，供您参考：
1. **《Functional Characterization of Recombinant Human Mitochondrial GPD2: Implications for Insulin Secretion》**
- 作者：Brown et al.
- 摘要：研究通过大肠杆菌系统成功表达并纯化人源GPD2重组蛋白，证实其在β细胞线粒体中的氧化活性对葡萄糖刺激的胰岛素分泌具有调控作用，为糖尿病机制提供新见解。
2. **《Cloning and Expression of Mouse GPD2 in Yeast: Role in Glycerol Metabolism》**
- 作者：Zhang & Chen
- 摘要：报道了小鼠GPD2基因在酿酒酵母中的异源表达，发现重组蛋白显著增强酵母甘油合成能力，揭示了GPD2在跨物种甘油磷酸穿梭中的保守功能。
3. **《Purification and Kinetic Analysis of Recombinant GPD2 from Drosophila melanogaster》**
- 作者：Itoh et al.
- 摘要：利用昆虫细胞表达系统获得高活性果蝇GPD2重组蛋白，酶动力学实验显示其对NAD+的特异性结合能力，为研究线粒体代谢酶进化提供数据支持。
注：上述文献为模拟示例，实际研究中建议通过PubMed或Web of Science以“GPD2 recombinant protein”等关键词检索最新文献。
**Background of GPD2 Recombinant Protein**
Glycerol-3-phosphate dehydrogenase 2 (GPD2), also known as mitochondrial glycerol-3-phosphate dehydrogenase, is a key enzyme involved in cellular energy metabolism. Located in the mitochondrial inner membrane, GPD2 catalyzes the reversible conversion of glycerol-3-phosphate (G3P) to dihydroxyacetone phosphate (DHAP), linking cytosolic glycolysis with mitochondrial oxidative phosphorylation. This reaction is critical for the glycerol phosphate shuttle, a mechanism that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, supporting ATP production under conditions of high metabolic demand.
The GPD2 gene, located on chromosome 2q24.3 in humans, encodes a flavin adenine dinucleotide (FAD)-dependent dehydrogenase. Dysregulation of GPD2 has been implicated in metabolic disorders, including obesity, diabetes, and non-alcoholic fatty liver disease, due to its role in lipid synthesis, insulin secretion, and redox balance. Additionally, GPD2 overexpression has been observed in certain cancers, where it may promote tumor growth by enhancing anabolic pathways.
Recombinant GPD2 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) for functional studies, drug screening, and structural analyses. Its purified form enables researchers to investigate enzyme kinetics, substrate specificity, and inhibitor interactions, aiding the development of therapies targeting metabolic diseases or cancer. Advances in protein engineering have also improved the stability and activity of recombinant GPD2. making it a valuable tool for elucidating mitochondrial dysfunction mechanisms and designing precision medicine strategies.
Overall, GPD2 serves as a crucial metabolic node, and its recombinant form provides a versatile platform for both basic research and therapeutic exploration.
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