首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BIO3 |
| Uniprot No | P50277 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-480aa |
| 氨基酸序列 | MSQEISYTPDVAELLDFDKKHIWHPYTSLSSPLNVYPVKSAHGCKLVLDTDSPVDVEVIDAMSSWWCVIHGYNNPELNEALTKQMLKFSHVLLGGFTHKGAVNLVQKLLKVIDEPSLQYCFLADSGSVAVEVALKMALQSNMSGEATKNRTKFLTIKNGYHGDTFGAMSVCDPENSMHHIYNDRLSENIFAQAPSIVDGLPTSQNGFEDHWNAEEVTDLKKQFELHSDKICAVILEPILQGAGGLRPYHPQFLIEVQKLCNQYDVLFIMDEIATGFGRTGEIFAFKHCQKYQDQHGISPSDQIKVVPDILCVGKGLTSGYMTMSAVVVNDKVASRISSPNSPTGGCFMHGPTFMGNALACSVAEKSMDILLRGEWRKQVSAIENQIYRELYQYIKNPDNGLIGTVVKRVSVIGAVGIVELYKKTDPEWFQKKFISKGVHIRPFNCLCYIMPPYVITTEELTKVNQVLIEVLHEWKSHINQ |
| 预测分子量 | 58.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BIO3重组蛋白的**模拟示例参考文献**(注：以下文献为假设，实际文献需通过学术数据库检索)：
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1. **文献名称**：*High-yield Expression and Purification of Recombinant BIO3 Protein in Escherichia coli*
**作者**：Chen L, Wang X, et al.
**摘要**：本研究优化了BIO3基因在大肠杆菌中的表达条件，利用亲和层析技术纯化获得高纯度蛋白，并通过Western blot验证其抗原性，为后续功能研究奠定基础。
2. **文献名称**：*Crystal Structure and Enzymatic Activity of BIO3: Implications for Biotin Biosynthesis*
**作者**：Müller R, Schmidt T, et al.
**摘要**：首次解析了BIO3蛋白的晶体结构，揭示了其在生物素合成途径中的催化机制，并通过体外酶活实验验证其底物特异性。
3. **文献名称**：*Functional Characterization of Recombinant BIO3 in Plant Stress Resistance*
**作者**：Gupta S, Patel M, et al.
**摘要**：通过转基因植物模型证明，BIO3重组蛋白能够增强植物对氧化应激的耐受性，提示其在农业生物技术中的潜在应用价值。
4. **文献名称**：*BIO3 Recombinant Protein as a Novel Therapeutic Target for Metabolic Disorders*
**作者**：Kim J, Park S, et al.
**摘要**：在小鼠模型中评估BIO3蛋白对脂代谢的调控作用，发现其通过AMPK信号通路改善肥胖表型，为代谢性疾病治疗提供新策略。
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如需真实文献，建议使用关键词 **"BIO3 recombinant protein"** 或 **"BIO3 gene expression"** 在 **PubMed**、**Web of Science** 或 **Google Scholar** 中检索，并筛选近5年内的研究以获取最新进展。
BIO3 recombinant protein is a engineered therapeutic protein developed through advanced genetic engineering techniques. Designed to address unmet medical needs in areas such as oncology, autoimmune disorders, or infectious diseases, it typically combines functional domains from native human proteins or antibodies with structural modifications to enhance stability, target specificity, and pharmacokinetics. The design often incorporates fusion protein strategies or site-directed mutagenesis to optimize receptor binding affinity while minimizing immunogenicity.
Produced in heterologous expression systems like Chinese Hamster Ovary (CHO) cells or *E. coli*, BIO3 undergoes post-translational modifications critical for biological activity. Its production process involves codon optimization, vector construction, and multi-step purification using affinity chromatography and size-exclusion methods. Rigorous quality control assays (e.g., ELISA, Western blot, endotoxin testing) ensure batch consistency and compliance with regulatory standards.
Mechanistically, BIO3 may function as a cytokine inhibitor, immune checkpoint modulator, or pathogen-neutralizing agent, depending on its intended application. Preclinical studies typically demonstrate improved efficacy over existing biologics in disease-specific models, with enhanced half-life through Fc fusion or PEGylation strategies. As a research tool, it facilitates studies on signaling pathways and protein-protein interactions. Current development phases focus on balancing therapeutic potency with manufacturability, addressing challenges in thermal stability and aggregation-prone regions through formulation optimization. BIO3 represents a convergence of structural biology insights and bioprocessing innovations in next-generation biologic development.
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