| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | arcA |
| Uniprot No | P41141 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-409aa |
| 氨基酸序列 | SVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTPWYYDPAMKMPIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKEVEFKRIVAINVPKWTNLMHLDTWLTMLDKNKFLYSPIANDVFKFWDYDLVNGGAEPQPQLNGLPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVKW |
| 预测分子量 | 48.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ArcA重组蛋白的3篇代表性文献及摘要概括：
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1. **文献名称**: *"Purification and characterization of the ArcA regulatory protein in Escherichia coli"*
**作者**: Iuchi S, Lin EC
**摘要**: 该研究报道了大肠杆菌ArcA重组蛋白的纯化及功能验证，利用表达载体在宿主细胞中过量表达ArcA，并通过磷酸化实验证明ArcA作为双组分系统的应答调控因子，参与厌氧代谢途径的调控。
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2. **文献名称**: *"Role of the ArcA regulator in the anaerobic adaptation of Salmonella Typhimurium"*
**作者**: Bang IS, Frye JG, McClelland M
**摘要**: 通过构建重组ArcA蛋白并敲除突变体，研究揭示了ArcA在沙门氏菌厌氧条件下的全局调控作用，包括对毒力基因和能量代谢相关基因的调控机制。
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3. **文献名称**: *"Structural insights into the DNA-binding domain of the ArcA response regulator"*
**作者**: Jeong JY, Kim HJ, Jung C
**摘要**: 利用重组ArcA蛋白的晶体结构解析，阐明了其DNA结合域的关键氨基酸残基如何识别靶启动子序列，为ArcA介导的转录抑制机制提供了分子基础。
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以上文献涵盖ArcA重组蛋白的功能验证、调控机制及结构分析，均为该领域的经典或关键研究。如需具体发表年份或期刊，可进一步补充检索。
The ArcA (Aerobic respiration control protein A) recombinant protein is derived from the ArcA/ArcB two-component regulatory system, primarily studied in *Escherichia coli* and related Gram-negative bacteria. This system enables bacterial adaptation to fluctuating oxygen levels, particularly during transitions between aerobic and anaerobic environments. ArcA functions as a response regulator, activated via phosphorylation by the sensor kinase ArcB under reducing conditions. Once phosphorylated (ArcA-P), it modulates the expression of numerous genes involved in metabolic pathways, including glycolysis, the tricarboxylic acid (TCA) cycle, and electron transport chains, to optimize energy production under oxygen-limited conditions.
Recombinant ArcA protein is engineered for in vitro studies to elucidate its structural and functional mechanisms. Produced through heterologous expression systems (e.g., *E. coli*), the protein is purified using affinity tags (e.g., His-tag) for experimental consistency. Researchers utilize recombinant ArcA to investigate its DNA-binding specificity, phosphorylation dynamics, and interaction with RNA polymerase or target promoters. Structural analyses, such as X-ray crystallography or cryo-EM, aim to resolve conformational changes during activation. Additionally, it serves as a tool to study cross-regulatory networks with other global regulators (e.g., Fnr or Cra) and its role in bacterial virulence, biofilm formation, or antibiotic resistance in pathogenic strains. The development of ArcA recombinant protein has advanced understanding of bacterial redox sensing and may inform strategies targeting bacterial metabolism for therapeutic applications.
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