| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXG1 |
| Uniprot No | P55316 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-489aa |
| 氨基酸序列 | MLDMGDRKEVKMIPKSSFSINSLVPEAVQNDNHHASHGHHNSHHPQHHHHHHHHHHHPPPPAPQPPPPPQQQQPPPPPPPAPQPPQTRGAPAADDDKGPQQLLLPPPPPPPPAAALDGAKADGLGGKGEPGGGPGELAPVGPDEKEKGAGAGGEEKKGAGEGGKDGEGGKEGEKKNGKYEKPPFSYNALIMMAIRQSPEKRLTLNGIYEFIMKNFPYYRENKQGWQNSIRHNLSLNKCFVKVPRHYDDPGKGNYWMLDPSSDDVFIGGTTGKLRRRSTTSRAKLAFKRGARLTSTGLTFMDRAGSLYWPMSPFLSLHHPRASSTLSYNGTTSAYPSHPMPYSSVLTQNSLGNNHSFSTANGLSVDRLVNGEIPYATHHLTAAALAASVPCGLSVPCSGTYSLNPCSVNLLAGQTSYFFPHVPHPSMTSQSSTSMSARAASSSTSPQAPSTLPCESLRPSLPSFTTGLSGGLSDYFTHQNQGSSSNPLIH |
| 预测分子量 | 53.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXG1重组蛋白的3篇示例参考文献(注：文献信息为虚构示例，建议通过PubMed或Google Scholar检索真实文献)：
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1. **标题**: *"Production and Functional Characterization of Recombinant FOXG1 Protein in E. coli"*
**作者**: Smith J. et al.
**摘要**: 研究报道了通过大肠杆菌表达系统高效生产重组FOXG1蛋白的方法，验证了其DNA结合活性及在体外调控靶基因转录的能力。
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2. **标题**: *"FOXG1 Recombinant Protein Rescues Neuronal Deficits in a Mouse Model of Rett Syndrome"*
**作者**: Chen L. et al.
**摘要**: 利用重组FOXG1蛋白治疗Rett综合征小鼠模型，发现其能通过激活神经保护通路改善突触可塑性和认知功能。
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3. **标题**: *"Structural Insights into FOXG1-DNA Interaction Using Purified Recombinant Protein"*
**作者**: Kim H. et al.
**摘要**: 通过X射线晶体学解析重组FOXG1蛋白与DNA结合的复合物结构，揭示了其特异性识别DNA基序的关键氨基酸残基。
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**提示**：实际文献可通过关键词“FOXG1 recombinant protein expression/purification/function”在学术平台检索，重点关注其在大脑发育、基因调控或疾病治疗中的应用研究。
**Background of FOXG1 Recombinant Protein**
FOXG1 (Forkhead Box G1) is a transcription factor belonging to the forkhead box (FOX) protein family, characterized by a conserved DNA-binding "forkhead" domain. It plays a critical role in early brain development, particularly in the regulation of telencephalon formation, neurogenesis, and cortical patterning. FOXG1 is highly expressed in the developing forebrain, where it governs the balance between neuronal proliferation and differentiation. Mutations or dysregulation of the *FOXG1* gene are linked to severe neurodevelopmental disorders, such as FOXG1 syndrome (formerly known as congenital variant of Rett syndrome), characterized by microcephaly, intellectual disability, seizures, and motor impairments.
Recombinant FOXG1 protein is engineered using biotechnological methods, typically by expressing the *FOXG1* gene in bacterial, insect, or mammalian cell systems. This purified protein retains the functional domains necessary for DNA binding and transcriptional regulation. Researchers utilize recombinant FOXG1 to study its molecular interactions, transcriptional targets, and role in neurodevelopment. It serves as a tool to investigate disease mechanisms caused by *FOXG1* mutations and to screen potential therapeutic compounds.
The production of recombinant FOXG1 also supports advances in gene therapy and protein replacement strategies for FOXG1-related disorders. Challenges include ensuring proper post-translational modifications (e.g., phosphorylation) that regulate its activity and stability, which may require eukaryotic expression systems. Additionally, recombinant FOXG1 is used in stem cell research to direct neuronal differentiation, aiding in disease modeling and regenerative medicine.
Overall, FOXG1 recombinant protein is a vital resource for unraveling the molecular basis of brain development and disorders, offering potential pathways for therapeutic intervention in neurodevelopmental diseases.
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