| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPS4A |
| Uniprot No | Q9UN37 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437aa |
| 氨基酸序列 | MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCVQYLDRAEKLKDYLRSKEKHGKKPVKENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDFGQES |
| 预测分子量 | 53.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VPS4A重组蛋白的模拟参考文献示例(内容为虚构，仅供格式参考)：
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1. **文献名称**: *Structural Insights into VPS4A ATPase Activity and Its Role in ESCRT-III Disassembly*
**作者**: Smith J., et al.
**摘要**: 本研究通过体外重组表达人源VPS4A蛋白，结合X射线晶体学解析其ATP结合结构域的三维结构，揭示了VPS4A依赖ATP水解解聚ESCRT-III复合物的分子机制，为理解细胞分裂和病毒出芽过程提供了新视角。
2. **文献名称**: *VPS4A Dysfunction in Cancer: Recombinant Protein-Based Functional Analysis*
**作者**: Chen L., et al.
**摘要**: 作者利用重组VPS4A蛋白进行体外酶活实验，发现其ATP酶活性在多种肿瘤细胞系中显著降低，并通过突变实验证明VPS4A功能缺失导致ESCRT通路异常，可能促进肿瘤细胞的多极分裂和基因组不稳定性。
3. **文献名称**: *Recombinant VPS4A as a Tool for Studying HIV-1 Budding Inhibition*
**作者**: Gonzalez R., et al.
**摘要**: 该研究通过重组VPS4A蛋白与HIV-1 Gag蛋白的相互作用实验，证明VPS4A的显性负突变体可竞争性抑制病毒颗粒的释放，为开发抗病毒药物提供了潜在靶点。
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注：以上文献为模拟内容，实际研究中请通过PubMed、Google Scholar等平台检索真实发表的论文。
**Background of VPS4A Recombinant Protein**
VPS4A (Vacuolar Protein Sorting 4 Homolog A) is a member of the AAA+ (ATPases Associated with Diverse Cellular Activities) protein family, known for its critical role in cellular membrane trafficking and organelle biogenesis. It functions as a key regulator of the Endosomal Sorting Complex Required for Transport (ESCRT) pathway, which mediates essential processes such as multivesicular body (MVB) formation, cytokinesis, viral budding, and membrane repair. VPS4A utilizes ATP hydrolysis to disassemble and recycle ESCRT-III polymers, ensuring dynamic membrane remodeling.
Recombinant VPS4A protein is engineered for *in vitro* studies to dissect its structural and functional mechanisms. Researchers often express it in bacterial or mammalian systems, preserving its ATPase activity and oligomerization capacity. Its recombinant form enables exploration of interactions with ESCRT-III subunits (e.g., CHMP proteins) and cofactors like VTA1. shedding light on how VPS4A coordinates membrane fission events.
Dysregulation of VPS4A is linked to diseases, including cancer and neurodegenerative disorders. Mutations or altered expression may disrupt ESCRT-dependent processes, leading to defective cell division, impaired autophagy, or aberrant extracellular vesicle release. For example, VPS4A loss has been implicated in tumorigenesis and chemoresistance, while its role in HIV-1 budding highlights therapeutic potential against viral infections.
Studies using recombinant VPS4A also aim to develop small-molecule modulators targeting its ATPase cycle or ESCRT-III binding, offering avenues for treating ESCRT-related pathologies. Overall, VPS4A recombinant protein serves as a vital tool for unraveling cellular membrane dynamics and advancing translational research.
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