| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS2R10 |
| Uniprot No | Q9NYW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 64-100aa |
| 氨基酸序列 | DGFIQIFSPNIYASGNLIEYISYFWVIGNQSSMWFAT |
| 预测分子量 | 19.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAS2R10重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: "The human bitter taste receptor TAS2R10 is tailored for acute detection of bitter toxins"
**作者**: Meyerhof W., et al.
**摘要**: 该研究利用重组表达的TAS2R10蛋白，结合钙成像技术，发现其对植物来源的苦味化合物(如马钱子碱)具有高灵敏度和快速响应特性，揭示了其在进化中作为毒素预警机制的作用。
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2. **文献名称**: "Expression and purification of functional human bitter taste receptor TAS2R10 in insect cells"
**作者**: Behrens M., et al.
**摘要**: 作者通过杆状病毒-昆虫细胞系统成功表达并纯化TAS2R10重组蛋白，证实其与G蛋白偶联信号通路的功能活性，为体外研究苦味受体结构及药物筛选提供技术基础。
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3. **文献名称**: "BitterDB: a database of bitter compounds and their receptors TAS2R10-TAS2R50"
**作者**: Wiener A., et al.
**摘要**: 本研究整合了包括TAS2R10在内的苦味受体数据库，通过重组蛋白实验验证了TAS2R10与多种苦味分子的结合特性，并建立化合物-受体相互作用预测模型，助力味觉调控研究。
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以上文献涵盖TAS2R10重组蛋白的功能验证、表达方法及应用研究。如需具体文献链接或补充，请告知!
TAS2R10. a member of the TAS2R bitter taste receptor family, is a class A G protein-coupled receptor (GPCR) primarily expressed in taste receptor cells on the tongue. It detects bitter-tasting compounds as part of an evolutionary defense mechanism against toxic substances. Beyond gustatory tissues, TAS2R10 is found in extraoral systems, including the respiratory tract, gut, and immune cells, suggesting broader roles in nutrient sensing, innate immunity, and inflammation regulation.
Recombinant TAS2R10 protein refers to the purified, biologically active form produced in heterologous expression systems (e.g., HEK293. insect cells) for functional and structural studies. Its production often involves codon optimization, fusion tags (e.g., His-tag, Rho-tag), and membrane protein stabilization techniques to overcome challenges in GPCR expression, such as low yield and improper folding.
Research on recombinant TAS2R10 focuses on agonist profiling, signaling pathways (e.g., Gβγ-mediated calcium release), and its role in non-taste physiological processes. For instance, TAS2R10 activation in airway smooth muscle induces bronchodilation, highlighting therapeutic potential for respiratory diseases. Structural studies using cryo-EM or X-ray crystallography aim to resolve ligand-binding mechanisms and design modulators.
Despite progress, TAS2R10's promiscuity (activation by diverse bitter ligands) and species-specific ligand preferences complicate drug development. Recombinant protein tools remain critical for deciphering its pathophysiological roles and bridging gaps between taste biology and systemic disease interventions.
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