| WB | 1/500-1/1000 | Tag |
| IF | 咨询技术 | Tag |
| IHC | 咨询技术 | Tag |
| ICC | 1/100 - 1/1000 | Tag |
| FCM | 1/200 - 1/400 | Tag |
| Elisa | 1/10000 | Tag |
| Aliases | HA;HA tag;HA-tag |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Tag Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Tag |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HA Tag抗体的3篇代表性文献(内容基于学术知识概括，部分信息或为示例性虚构)：
1. **《A monoclonal antibody for influenza virus hemagglutinin detects HA-tagged proteins in Western blot》**
*作者：Wilson, I.A., et al. (1985)*
摘要：首次报道了针对流感病毒血凝素(HA)表位的单克隆抗体的开发，验证了其在Western blot中对HA标签融合蛋白的特异性识别，为后续标签系统应用奠定基础。
2. **《Epitope tagging in mammalian cells: A study of HA tag antibody utility in protein interaction assays》**
*作者：Roth, M.G., et al. (1996)*
摘要：系统评估了HA标签抗体在哺乳动物细胞中的适用性，证明其适用于免疫共沉淀(Co-IP)和免疫荧光(IF)，并优化了跨物种实验条件。
3. **《Comparative analysis of commercial HA antibodies reveals variability in sensitivity for low-abundance protein detection》**
*作者：Lee, S., & Zhang, Y. (2018)*
摘要：对比了多种商业化HA抗体的性能，发现不同来源(如小鼠单抗vs.兔多抗)的灵敏度差异显著，为实验中的抗体选择提供数据支持。
(注：以上文献标题及作者为示例性整合，如需真实文献建议通过PubMed/Google Scholar以关键词"HA tag antibody" + "application"检索近年高引论文。)
The HA (hemagglutinin) tag is a small epitope tag derived from the influenza virus hemagglutinin protein, corresponding to amino acids 98-106 (YPYDVPDYA). First developed in the late 1980s, HA Tag antibodies were engineered to detect and purify recombinant proteins in molecular and cell biology research. The tag’s compact size (9-12 amino acids) minimizes interference with protein structure or function, making it a versatile tool for tracking expressed proteins in vitro and in vivo.
HA Tag antibodies are typically monoclonal or polyclonal and exhibit high specificity and affinity for the HA epitope. They are widely used in techniques such as Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry to identify, localize, or isolate tagged proteins. Their utility is enhanced by compatibility with mammalian, bacterial, and insect expression systems, allowing cross-platform applications. Additionally, HA Tag systems often include standardized protocols and commercial availability, streamlining experimental workflows.
Compared to other epitope tags (e.g., FLAG, Myc), HA Tag antibodies are favored for their robust performance in detecting low-abundance proteins and adaptability to diverse experimental conditions. This reliability has cemented their role in studying protein interactions, post-translational modifications, and subcellular localization, contributing to advances in gene expression analysis, drug development, and functional proteomics.
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