关键信息
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基因名
VAPB
- 应用
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别名
ALS8; VAMP-B; VAMP-C; VAP-B; VAP-C
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O95292
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表达区间
Met1~Pro132
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分子量
19kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
VAPB (Vesicle-Associated Membrane Protein B) is a crucial protein involved in various cellular processes, including the regulation of lipid metabolism, endoplasmic reticulum (ER) function, and mitochondrial dynamics. Its significance is underscored by its association with neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA), where mutations in the VAPB gene have been linked to disease onset and progression. The protein is thought to play a role in maintaining cellular homeostasis by mediating the connection between the ER and other organelles, thus influencing intracellular signaling pathways and organelle communication. Recent studies have highlighted the importance of VAPB's oligomerization and interaction with other proteins in maintaining neuronal health. Researchers have turned to recombinant technology to produce VAPB proteins for in-depth functional studies and potential therapeutic applications. By generating and characterizing VAPB recombinant proteins, scientists aim to unravel the molecular mechanisms underlying its role in cellular processes and its implications in neurodegeneration, ultimately paving the way for novel therapeutic strategies targeting VAPB and related pathways in disease contexts.
