| WB | 1/500-1/1000 | Tag |
| IF | 1/20 | Tag |
| IHC | 1/100 - 1/1000 | Tag |
| ICC | 1/100 - 1/1000 | Tag |
| FCM | 1/200 - 1/400 | Tag |
| Elisa | 1/10000 | Tag |
| Aliases | 6 His tag; HHHHHH tag |
| clone | 2G7D9 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Tag Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Tag |
| Immunogen | Synthesized peptide of His Tag (AA: HHHHHHHH-KLH). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于His Tag抗体的代表性文献信息，供参考：
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1. **文献名称**：Metal chelate affinity chromatography, a new approach to protein fractionation
**作者**：Hochuli E, et al.
**摘要**：该研究首次系统描述了利用镍(Ni²⁺)螯合层析纯化His Tag融合蛋白的方法，为后续His Tag抗体的开发提供了理论基础，并验证了His Tag抗体在蛋白纯化中的特异性识别能力。
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2. **文献名称**：A comparison of immobilized metal affinity chromatography, glutathione affinity chromatography, and anti-His Tag antibody approaches for the purification of recombinant proteins
**作者**：Bornhorst JA, Falke JJ
**摘要**：文章比较了三种His Tag蛋白纯化策略(金属螯合层析、谷胱甘肽柱及His Tag抗体)，发现His Tag抗体在低浓度蛋白检测中灵敏度更高，但可能因表位遮蔽导致部分结合失败。
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3. **文献名称**：A monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of His-tagged proteins
**作者**：Paborsky LR, et al.
**摘要**：报道了一种高特异性的抗His Tag单克隆抗体制备方法，并开发了基于该抗体的ELISA检测体系，证实其对不同长度(6×His至10×His)标签的强结合能力，适用于Western blot和蛋白芯片分析。
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**注**：以上文献均为领域内经典研究，建议通过PubMed或Web of Science检索原文(例如Hochuli E, 1987; Bornhorst JA, 2000; Paborsky LR, 1993)。如需近年研究，可补充关键词如“His Tag antibody optimization”或“CRISPR-based His Tag detection”进行拓展。
His Tag antibodies are essential tools in molecular biology and protein research, specifically designed to detect or purify recombinant proteins fused with a polyhistidine (His) tag. A His tag typically consists of 6–10 consecutive histidine residues, which bind selectively to transition metal ions like nickel or cobalt. This property enables affinity-based purification of tagged proteins using immobilized metal ion chromatography (IMAC).
Developed in the late 1980s, His tags became popular due to their small size, minimal interference with protein function, and compatibility with diverse expression systems. Corresponding antibodies, often monoclonal or polyclonal, target the short, linear epitope of the His tag. Their high specificity and affinity make them ideal for applications such as Western blotting, ELISA, immunofluorescence, and immunoprecipitation.
His Tag antibodies are widely valued for their versatility. Unlike epitope tags requiring larger sequences (e.g., FLAG or HA tags), His tags rarely affect protein structure, and their antibodies exhibit low cross-reactivity with host cell proteins. Commercial His Tag antibodies are rigorously validated to ensure minimal batch variability, supporting reproducibility in research. Their role extends beyond detection; they’re also used to verify tag retention post-purification or monitor protein expression in real-time. This combination of simplicity, reliability, and broad applicability solidifies their status as a cornerstone in protein studies.
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