| WB | 1/500-1/1000 | Tag |
| IF | 1/20-1/200 | Tag |
| IHC | 1/100 - 1/1000 | Tag |
| ICC | 1/100 - 1/1000 | Tag |
| FCM | 1/200 - 1/400 | Tag |
| Elisa | 1/10000 | Tag |
| Aliases | 6 His tag; HHHHHH tag |
| clone | 6A8H8 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Tag Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Tag |
| Immunogen | Synthesized peptide of His Tag (AA: HHHHHHHH-KLH). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于His Tag抗体的参考文献，简要概括内容如下：
1. **《Immobilized metal ion affinity chromatography》**
作者：Hochuli, E., et al.
摘要：该文献首次系统介绍了Ni²⁺-NTA(镍-氮川三乙酸)层析技术，阐述了His标签蛋白与固定化金属离子的特异性结合原理，为His标签抗体在蛋白纯化中的应用奠定基础。
2. **《Comparison of Immobilized Metal Affinity Chromatography Resins》**
作者：Bornhorst, J.A., & Falke, J.J.
摘要：研究对比了不同金属离子(Ni²⁺、Co²⁺、Cu²⁺)配体层析树脂对His标签蛋白的纯化效果，分析了His Tag抗体在检测和纯化中的灵敏度及适用条件优化策略。
3. **《Tags for protein detection and purification》**
作者：Terpe, K.
摘要：综述性文章，详细比较了His标签与其他蛋白标签(如GST、FLAG)的优缺点，强调His Tag抗体在Western blot和ELISA中的高通用性及低交叉反应特性。
4. **《Optimization of His-Tagged Protein Purification in Mammalian Systems》**
作者：Kim, J., et al.
摘要：探讨在哺乳动物表达系统中使用His标签抗体进行蛋白纯化的优化方案，包括去垢剂选择、洗脱条件调整及抗体特异性验证方法，提升复杂样本中的检测准确性。
以上文献涵盖了His Tag抗体的核心原理、技术比较及实际应用优化，适合作为实验设计或论文撰写的参考依据。
His-tag antibodies are essential tools in molecular biology and protein research, primarily used to detect or purify recombinant proteins engineered with a polyhistidine (His) tag. The His-tag, typically a short sequence of 6–10 histidine residues, was introduced in the 1980s as an affinity tag due to its ability to bind metal ions like nickel or cobalt in immobilized metal affinity chromatography (IMAC). This property simplifies protein purification under native or denaturing conditions.
His-tag antibodies are produced by immunizing animals (e.g., mice, rabbits) with His-tagged proteins or synthetic peptides. These antibodies recognize the His-tag epitope with high specificity, enabling applications such as Western blotting, ELISA, immunofluorescence, and immunoprecipitation. Unlike IMAC-based purification, His-tag antibodies allow detection or capture of His-tagged proteins without requiring metal-coordinated interactions, making them versatile in diverse experimental conditions.
Both monoclonal and polyclonal His-tag antibodies are available. Monoclonal antibodies offer consistent specificity, while polyclonal versions may provide higher sensitivity due to multiple epitope recognition. A key advantage of His-tag antibodies is their compatibility with proteins from any host organism, as the His-tag is an artificial sequence. They are widely used in studying protein expression, localization, and interactions, particularly in systems where endogenous histidine-rich proteins are absent or minimal. Their reliability and broad applicability have solidified their role as a cornerstone reagent in proteomics and biotechnology workflows.
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