| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALKBH2 |
| Uniprot No | Q6NS38 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-261aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMDRFLVKGAQGGLLRKQEEQEPTGEEPAVL GGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADE IFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLS PKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAP GSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPT NTHWYHSLPVRKKVLAPRVNLTFRKILLTKK |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALKBH2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Human AlkB homolog ABH2 is an alkylation repair enzyme"*
**作者**：Duncan, T., et al.
**摘要**：该研究首次证实ALKBH2重组蛋白在体外可有效修复DNA中的1-甲基腺嘌呤和3-甲基胞嘧啶损伤，揭示了其在烷基化损伤修复中的核心作用，并证明其酶活性依赖于Fe(II)和α-酮戊二酸。
2. **文献名称**：*"Structural basis of ALKBH2-mediated direct DNA damage reversal"*
**作者**：Yang, G., et al.
**摘要**：通过晶体结构分析，研究解析了ALKBH2重组蛋白与DNA复合物的三维结构，阐明了其特异性识别和催化修复甲基化损伤的分子机制，并揭示了底物结合口袋的关键氨基酸残基。
3. **文献名称**：*"Substrate specificity of human ALKBH2 and involvement of the enzyme in repair of 3-methylcytosine lesions in DNA"*
**作者**：Aas, P.A., et al.
**摘要**：该文利用重组ALKBH2蛋白进行酶活测定，发现其优先修复双链DNA中的3-甲基胞嘧啶，且在人类细胞中通过碱基切除修复途径参与维持基因组稳定性。研究还比较了ALKBH2与其他AlkB家族成员的底物偏好性差异。
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**说明**：以上文献涵盖ALKBH2重组蛋白的功能验证(修复机制)、结构解析及底物特异性研究，均为该领域的经典工作。如需扩展，可进一步检索其在癌症或表观遗传学中的相关应用研究。
ALKBH2 (AlkB homolog 2) is a human DNA/RNA repair enzyme belonging to the AlkB family of non-heme Fe(II)/α-ketoglutarate-dependent dioxygenases. It plays a critical role in maintaining genomic stability by repairing alkylation damage, particularly targeting mutagenic lesions such as 1-methyladenine (1mA) and 3-methylcytosine (3mC) in DNA. Discovered as a functional homolog of the E. coli AlkB protein, ALKBH2 operates through oxidative demethylation, converting alkylated bases back to their unmodified forms. This corrective mechanism is vital for counteracting alkylating agents from environmental toxins or chemotherapeutic drugs.
Recombinant ALKBH2 protein is produced via genetic engineering, typically expressed in bacterial (e.g., E. coli) or mammalian systems to ensure proper folding and enzymatic activity. Purification often involves affinity tags (e.g., His-tag) for standardized isolation. Its recombinant form enables detailed biochemical studies, including substrate specificity, kinetic analysis, and structural characterization via X-ray crystallography or cryo-EM. Researchers utilize it to investigate DNA repair pathways, epigenetic regulation (via potential RNA demethylation activity), and cancer biology, as ALKBH2 overexpression correlates with tumor progression in certain cancers.
Additionally, ALKBH2 recombinant protein has applications in drug discovery, serving as a target for inhibitors aimed at sensitizing cancer cells to alkylating therapies. Its role in repairing chemotherapy-induced DNA damage makes it both a therapeutic target and a tool for understanding cellular resistance mechanisms. Studies also explore its interplay with other repair proteins and potential involvement in neurodegenerative diseases linked to DNA damage accumulation.
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