| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX1 |
| Uniprot No | Q92499 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-740aa |
| 氨基酸序列 | MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRATKGLMKGKHYYEVSCHDQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEIPPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQVTQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLSVLENGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF |
| 预测分子量 | 89.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DDX1重组蛋白研究的3篇代表性文献摘要示例(请注意文献信息为示例，实际引用请通过学术数据库核实)：
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1. **文献名称**: *"Biochemical characterization of the human DEAD-box protein DDX1"*
**作者**: Liu, Z., et al.
**摘要**: 本研究通过原核表达系统(大肠杆菌)纯化了重组人源DDX1蛋白，分析了其ATP酶活性和RNA解旋酶功能。发现DDX1在体外依赖ATP水解可解开双链RNA结构，并揭示了其与RNA结合域的协同作用机制。
2. **文献名称**: *"DDX1 interacts with the RNA-dependent RNA polymerase of SARS-CoV-2 and enhances viral replication"*
**作者**: Wang, R., et al.
**摘要**: 通过重组DDX1蛋白与新冠病毒RdRp的体外互作实验，发现DDX1通过结合病毒RNA聚合酶促进病毒RNA的复制效率，揭示了DDX1在宿主-病毒相互作用中的关键角色。
3. **文献名称**: *"Structural insights into the dsRNA recognition by DDX1 helicase"*
**作者**: Chen, Y., et al.
**摘要**: 利用重组DDX1蛋白进行X射线晶体学研究，解析了其结合双链RNA的构象变化，阐明了DDX1通过特定结构域识别RNA二级结构的分子机制，为设计靶向DDX1的抗病毒药物提供结构基础。
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**注意**：以上文献信息为示例性质，实际研究中请通过PubMed、Google Scholar等平台检索最新文献(关键词：DDX1 recombinant protein, DEAD-box helicase DDX1)。DDX1相关研究多集中在RNA代谢调控、肿瘤耐药性及病毒感染机制领域，重组蛋白技术常用于其生化功能及互作蛋白分析。
**Background of DDX1 Recombinant Protein**
DDX1. a member of the DEAD-box RNA helicase family, is a conserved ATP-dependent enzyme critical for RNA metabolism, including splicing, transport, translation, and degradation. It plays roles in genomic stability, viral replication, and stress responses. Structurally, DDX1 contains conserved helicase domains that enable RNA binding and unwinding. Dysregulation of DDX1 is linked to cancers, neurodevelopmental disorders, and neurodegenerative diseases, highlighting its biomedical significance.
Recombinant DDX1 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling controlled production for functional studies. Purification methods, such as affinity chromatography, ensure high purity and activity. Recombinant DDX1 facilitates *in vitro* studies on RNA-protein interactions, helicase activity, and its role in viral replication (e.g., HIV-1). It also serves as a tool for screening therapeutic inhibitors and probing structural mechanisms via crystallography or cryo-EM.
Research using recombinant DDX1 has advanced understanding of its nucleic acid remodeling functions and potential as a drug target, underscoring its value in both basic and applied biomedical research.
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