| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FERMT2 |
| Uniprot No | Q96AC1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-680aa |
| 氨基酸序列 | MALDGIRMPDGCYADGTWELSVHVTDLNRDVTLRVTGEVHIGGVMLKLVEKLDVKKDWSDHALWWEKKRTWLLKTHWTLDKYGIQADAKLQFTPQHKLLRLQLPNMKYVKVKVNFSDRVFKAVSDICKTFNIRHPEELSLLKKPRDPTKKKKKKLDDQSEDEALELEGPLITPGSGSIYSSPGLYSKTMTPTYDAHDGSPLSPTSAWFGDSALSEGNPGILAVSQPITSPEILAKMFKPQALLDKAKINQGWLDSSRSLMEQDVKENEALLLRFKYYSFFDLNPKYDAIRINQLYEQAKWAILLEEIECTEEEMMMFAALQYHINKLSIMTSENHLNNSDKEVDEVDAALSDLEITLEGGKTSTILGDITSIPELADYIKVFKPKKLTLKGYKQYWCTFKDTSISCYKSKEESSGTPAHQMNLRGCEVTPDVNISGQKFNIKLLIPVAEGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNLEVQNILSFLKMQHLNPDPQLIPEQITTDITPECLVSPRYLKKYKNKQITARILEAHQNVAQMSLIEAKMRFIQAWQSLPEFGITHFIARFQGGKKEELIGIAYNRLIRMDASTGDAIKTWRFSNMKQWNVNWEIKMVTVEFADEVRLSFICTEVDCKVVHEFIGGYIFLSTRAKDQNESLDEEMFYKLTSGWV |
| 预测分子量 | 83.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FERMT2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: "FERMT2 (Kindlin-2) regulates integrin activation and tumor progression in pancreatic cancer"
**作者**: Yu Y, et al.
**摘要**: 本研究探讨了FERMT2(KINDIN-2)重组蛋白在胰腺癌中的作用，发现其通过调控整合素信号通路激活β1-integrin，促进癌细胞迁移和侵袭。重组FERMT2蛋白的体外实验表明其通过结合ILK(整合素连接激酶)增强细胞外基质黏附能力。
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2. **文献名称**: "Structural insights into the interaction of FERMT2 with the cytoplasmic tail of β-integrin"
**作者**: Li H, et al.
**摘要**: 通过X射线晶体学解析了重组人源FERMT2蛋白与β-integrin胞内结构域的复合物结构，揭示了FERMT2的F3结构域通过特定氨基酸残基与整合素结合，为设计靶向FERMT2-整合素相互作用的药物提供了结构基础。
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3. **文献名称**: "FERMT2 knockdown impairs neuronal morphogenesis and promotes Alzheimer’s disease pathology"
**作者**: Xiao S, et al.
**摘要**: 研究利用重组FERMT2蛋白在神经元模型中的功能实验，发现其缺失导致tau蛋白异常磷酸化和轴突发育缺陷，提示FERMT2在阿尔茨海默病中可能通过调控细胞骨架稳定性影响病理进程。
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**备注**：以上文献信息为示例，实际文献需通过PubMed或Web of Science等平台检索确认。若需具体论文链接或补充文献，可进一步提供关键词筛选。
FERMT2. also known as Kindlin-2. is a cytoskeletal protein encoded by the *FERMT2* gene, belonging to the Fermitin family. It plays a critical role in integrin activation, cell-matrix adhesion, and cell signaling by linking integrins to the actin cytoskeleton. As a key regulator of integrin-mediated processes, FERMT2 is essential for cellular functions such as migration, proliferation, and tissue morphogenesis. Its involvement in pathways like Wnt/β-catenin and TGF-β signaling further underscores its importance in embryonic development, wound healing, and tissue homeostasis.
Recombinant FERMT2 protein is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or mammalian expression systems, followed by purification steps like affinity chromatography. This engineered protein retains the functional domains of native FERMT2. including the FERM (F for 4.1 protein, E for ezrin, R for radixin, and M for moesin) domain, which mediates interactions with integrins and cytoskeletal proteins. Researchers utilize recombinant FERMT2 to study its molecular interactions, structural properties, and role in diseases. Notably, *FERMT2* polymorphisms are linked to Alzheimer’s disease risk, and aberrant FERMT2 expression is implicated in cancer metastasis, fibrosis, and inflammatory disorders. Recombinant forms enable drug screening, mechanistic studies, and therapeutic development targeting FERMT2-associated pathways. Its applications extend to disease modeling, biomarker discovery, and exploring tissue regeneration mechanisms.
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