| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIEZO1 |
| Uniprot No | Q92508 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2198-2431aa |
| 氨基酸序列 | RSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPQPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKVSPPS |
| 预测分子量 | 33.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PIEZO1重组蛋白的关键文献概览：
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1. **文献名称**：*Structure of the mechanically activated ion channel Piezo1*
**作者**：Ge, J., et al. (2015)
**摘要**：该研究首次通过冷冻电镜解析了重组表达的小鼠PIEZO1蛋白的三维结构，揭示了其独特的三叶螺旋桨状构象，为理解机械力感知机制提供了结构基础。研究使用HEK293细胞重组表达并纯化蛋白。
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2. **文献名称**：*Piezo1 integration of vascular architecture with physiological force*
**作者**：Li, J., et al. (2014)
**摘要**：通过在哺乳动物细胞中重组表达人源PIEZO1蛋白，研究发现其介导内皮细胞对流体剪切力的响应，调控血管发育。实验结合基因敲除和功能分析，证实重组PIEZO1的机械敏感性在血管形成中的关键作用。
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3. **文献名称**：*Yoda1 analogue (Dooku1) which antagonizes Yoda1-evoked activation of Piezo1*
**作者**：Syeda, R., et al. (2015)
**摘要**：利用重组表达的PIEZO1蛋白进行高通量筛选，发现小分子Yoda1可特异性激活通道，并开发其拮抗剂Dooku1.研究验证了重组蛋白在药物筛选中的应用潜力。
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**注**：以上文献均涉及重组PIEZO1蛋白的表达与功能验证，涵盖结构解析、生理机制及药物开发方向。如需具体DOI或补充文献，可进一步提供。
PIEZO1 is a mechanosensitive ion channel protein that plays a critical role in converting mechanical stimuli into cellular electrochemical signals. Discovered in 2010 as part of the Piezo protein family, PIEZO1 is a large, transmembrane protein composed of ~2.500 amino acids. It forms a unique three-bladed propeller-like homotrimeric structure, which enables it to sense mechanical forces such as membrane tension, shear stress, or pressure changes. This mechanotransduction capability is vital for numerous physiological processes, including vascular development, red blood cell volume regulation, and tactile sensation.
Recombinant PIEZO1 proteins are engineered versions produced in heterologous expression systems (e.g., HEK293 cells) for structural and functional studies. These proteins retain the native channel's ability to open in response to mechanical cues, allowing researchers to investigate its gating mechanisms, ion selectivity, and interactions with signaling molecules. The production of recombinant PIEZO1 has been pivotal in overcoming challenges associated with studying endogenous Piezo channels, which are often expressed at low levels in native tissues.
Research on recombinant PIEZO1 has unveiled its involvement in diseases. Gain-of-function mutations are linked to hereditary xerocytosis (a hemolytic anemia), while loss-of-function variants correlate with lymphatic dysplasia. Its role in pathologies like hypertension and atherosclerosis has also been explored. Despite progress, challenges remain in purifying full-length PIEZO1 due to its size (>300 kDa per subunit) and structural complexity. Advances in cryo-EM and electrophysiological techniques, however, have enabled breakthroughs in understanding its 3D architecture and activation mechanisms. Recombinant PIEZO1 continues to drive therapeutic innovation, serving as a target for drug development aimed at modulating mechanosensitive pathways in disease.
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